葛根素和黄芪甲苷及其配伍对T2DM大鼠的影响  

Regulation of puerarin and astragaloside A on rats modeled with type 2 diabetes mellitus

作　　者：田震亚[1] 王会敏[1] 田炜[1] 喇孝瑾[1] 高秀娟[1] 韩婷[1] 王亚[1] Hegyi Gabriella 陈震 李继安[1] 

机构地区：[1]河北联合大学中医学院,河北唐山063000 [2]匈牙利佩奇大学 [3]匈牙利东方国药集团

出　　处：《河北联合大学学报（医学版）》2013年第4期453-455,共3页Journal of North China Coal Medical College

基　　金：科技部2011~2012年度中国与匈牙利政府间科技合作项目(编号:2010-5-8);唐山市科技局项目(编号:08360202A-8)

摘　　要：①目的观察葛根素、黄芪甲苷及其配伍对2型糖尿病大鼠血糖、血脂、超敏C反应蛋白(hs-CRP)及胰腺病理组织学的影响,初步探讨其作用机制。②方法以高糖、高脂饮食+链脲佐菌素腹腔注射法建立2型糖尿病大鼠模型,随机分为模型组、二甲双胍组、葛根素组、黄芪甲苷组、葛根素+黄芪甲苷组。治疗前及治疗2、4、6周分别测定各组大鼠空腹血糖(FBG)及餐后2小时血糖(P2hPG),并于给药6周后,测定大鼠CHOL、TG、HDL-C、LDL-C、hs-CRP含量,光镜下观察胰腺病理组织学变化。③结果各治疗组大鼠FBG、P2hPG、CHOL、TG、LDL-C、hs-CRP较M组降低,HDL-C含量增加,对胰腺胰岛细胞损伤有不同程度改善。PA组LDL-C水平较P组、A组降低明显(P<0.05),对胰腺胰岛细胞损伤明显改善。④结论葛根素、黄芪甲苷及其配伍可改善实验性2型糖尿病大鼠的糖、脂代谢,其作用机制可能抑制炎症反应有关。其中葛根素配伍黄芪甲苷在降低LDL-C水平、改善胰岛细胞结构方面优于单纯葛根素、黄芪甲苷。Objective To observe effects of puerarin and astragaloside A on blood glucose,blood lipid,hs-CRP and pancreatic histopathology in rats modeled with type 2 diabetes mellitus（T2DM）,and to approach their pharmacological mechanism.Methods T2DM rats that were induced by high calories diet and intraperitoneal injection of streptozotacin（STZ） were randomly divided into five groups： diabetes mellitus group（M）,melbine group（D）,puerarin group（P）,astragaloside A group（A） and puerarin with astragaloside A group（PA）.Fasting blood glucose（FBG） and 2-hour postprandial blood glucose（P2hPG） of rats in each group were measured before treatment and 2,4,6 weeks.After six weeks of administration of the medicine,blood was extracted for measurement on blood glucose,as well as following indexes,CHOL,TG,HDL-C,LDL-C,hs-CRP.Pathological changes were observed under light microscope by HE staining.Results All indexes were inhibited to certain degrees in every treatment group except HDL-C.The level of LDL-C in PA group decreased more significantly than that in the P group and A group（P ＆lt; 0.05）,meanwhile,the injury pancreas tissue was improved significantly.Conclusion The puerarin and Astragaloside A could markedly improved glucose and lipid metabolism in experimental type 2 diabetic rats.It can be infered that puerarin and astragaloside A can relieve suppressing inflammatory reaction.In terms of decrease of LDL-C and improvement of the structure of islet cells,the effects in PA group were more stronger than those in P and A group.

关 键 词：2型糖尿病 葛根素 黄芪甲苷 血糖 血脂 

分 类 号：R29[医药卫生—民族医学]