甘草酸苷体外上调乙肝疫苗无应答者免疫功能  

Glycyrrhizin up-regulate immune function of non-responders to hepatitis B vaccine in vitro

作　　者：李旭华 陈月 吴文苑 陈茳晶[1,2] 金红涛 

机构地区：[1]暨南大学第二临床医学院,深圳市人民医院检验科,深圳518020 [2]香港大学深圳医院病理科,深圳518052

出　　处：《中国免疫学杂志》2013年第7期718-722,共5页Chinese Journal of Immunology

基　　金：广东省自然科学基金(07300964)资助

摘　　要：目的:研究甘草酸苷(GL)对乙肝疫苗无应答者免疫功能的影响,为使乙肝疫苗应答率得到有效提升提供一种可行的方法。方法:分离研究对象PBMC,加入HBsAg和(或)GL的条件下体外培养,Real-time PCR测定刺激后IFN-γ、IL-4、IL-10、CD80、CD86 mRNA的表达水平,并与对照组和应答组比较。收集非贴壁细胞用于培养CTL,贴壁细胞诱导分化为DC;CCK-8法检测各组DC刺激同种异体淋巴细胞增殖的能力及DC激活的CTL细胞对K562细胞的杀伤作用。结果:HBsAg+GL联合刺激组PBMC的IFN-γ、IL-10、CD80、CD86 mRNA表达水平高于HBsAg单独刺激组,差异均有统计学意义(P<0.05),但IFN-γmRNA表达低于应答组(P<0.05),IL-10、CD80 mRNA无明显差异(P>0.05),而CD86 mRNA表达高于应答组(P<0.05);IL-4 mRNA表达无明显变化;与HBsAg单独刺激组比较,HBsAg+GL联合刺激组DC刺激同种异体淋巴细胞增殖的作用明显增强(P<0.05),且高于应答组(P<0.05),其激活的CTL对K562细胞杀伤作用亦显著提高(P<0.05),接近应答组水平(P>0.05)。结论:GL对乙肝疫苗无应答者免疫功能具有调节作用,联合HBsAg有可能诱导机体创造更有利于乙肝保护性抗体产生的环境,为其临床应用提供实验依据。Objective：To observe the effects of Glycyrrhizin（GL） on the immune function of non-responders to hepatitis B vaccine and provide a available method to effectively enhance the response rate of hepatitis B vaccine.Methods： PBMC were isolated from research targets with incubation of GL and（or） HBsAg.The mRNA expression levels of IFN-γ,IL-4,IL-10,CD80,CD86 were detected by Real-time PCR and compared with control group and response group.Collect non-adherent cells for culturing CTL cells,and adherent cells induced to differentiate into DCs;the ability to stimulate the proliferation of allogenic T cells by DCs and the ability to kill K562 cells by CTL cells were detected by CCK-8.Results： The mRNA expression levels of IFN-γ,IL-10,CD80,CD86 in HBsAg ＋ GL ＋ non-responder group were significantly higher than those in HBsAg＋non-responders group（P0.05）.But IFN-γ mRNA expression level was lower than that of response group（P0.05）,IL-10 and CD80 mRNA expression levels had no obvious differences（P0.05）,and CD86 mRNA expression level was higher than that of response group（P0.05）;IL-4 mRNA expression level had no obvious changes.Compared with DC of HBsAg stimulating group,the ability of DC of HBsAg ＋ GL stimulating group to stimulate proliferation of allogenic T cells was significantly enhanced（P0.05）,and higher than that of response group.The CTL cells induced by DCs of HBsAg ＋ GL stimulating group had stronger cytotoxicity against K562 cells（P0.05）,and tend to the level of response group（P0.05）.Conclusion： GL can regulate the immune function of non-responders to hepatitis B vaccine,HBsAg ＋ GL stimulating may induce the body to create a environment conducive to the generation of protective antibodies for hepatitis B virus,which provide an experimental basis for its clinical application.

关 键 词：乙肝疫苗 无应答 甘草酸苷 免疫功能 

分 类 号：R392.1[医药卫生—免疫学]