水稻蜡质合成相关基因OsGL1-5的表达模式及逆境响应特征分析  

Expression Pattern and Stress Responses of Wax Biosynthesis Related Gene OsGL1-5 in rice

作　　者：周玲艳[1] 倪尔冬 朱丽雅[1] 梁红[1] 庄楚雄[2] 

机构地区：[1]仲恺农业工程学院生命科学学院,广东广州510225 [2]华南农业大学生命科学学院,广东广州510642

出　　处：《核农学报》2013年第7期938-945,共8页Journal of Nuclear Agricultural Sciences

基　　金：广东省自然科学基金(S2011040001653)

摘　　要：通过生物信息学方法对水稻蜡质合成相关基因OsGL1-5启动子序列特点进行分析;并以水稻中花11不同组织和发育时期的穗子为材料,通过半定量RT-PCR及mRNA原位杂交技术分析OsGL1-5的时空表达特征;同时,分别以200mmol·L-1NaCl、100μmol·L-1ABA和1.0%H2O2处理水稻幼苗,通过半定量RT-PCR分析逆境胁迫下OsGL1-5的表达模式。生物信息学分析表明,OsGL1-5启动子中包括大量与干旱、冷、盐等多种逆境胁迫相关的调控序列。时空表达特征分析表明,OsGL1-5主要在不同发育时期的穗子中表达,且表达部位集中在稃片的毛状体和花药绒毡层中,叶中表达量相对较低,而茎和根中没有检测到表达。NaCl、ABA以及H2O2等逆境胁迫下,OsGL1-5表达量在不同处理时间有所增加。In this study,the promoter of wax biosynthesis related gene OsGL1-5 in rice was analyzed via bioinformatics,and the temporal and spatial expression of OsGL1-5 was analyzed by semi-quantitative RT-PCR and mRNA in situ hybridization using the different tissues and developmental stage panicles as materials.In addition,the expression of OsGL1-5 was analyzed by semi-quantitative RT-PCR when the seedlings were treated with stresses,including 200mmol·L-1 NaCl,100μmol·L-1 ABA and 1.0% H2O2.The bioinformatics analysis showed that the promoter of OsGL1-5（about 2 kb upstream of OsGL1-5） was predicted to contain important regulatory elements including drought,high salt,coldness,ABA,light regulation,copper,and oxygen responsive elements.The analysis of OsGL1-5 expression profiles revealed that OsGL1-5 was expressed highly in panicles at different developmental stages and specifically expressed in the trichomes of glume and the tapetum of anther,low expression of OsGL1-5 was also detected in the leaves,however,its expression was not detected in the stems and roots.The analysis of OsGL1-5 in response to stresses showed that the expression of OsGL1-5 was increased at different time when the seedlings were treated with NaCl,ABA and H2O2.

关 键 词：水稻 OsGL1-5 表达 逆境 

分 类 号：S511[农业科学—作物学]