正交设计法优选盾叶薯蓣胚性愈伤诱导的实验研究  

Study on Inducing Embryogenic Callus of Dioscorea zingiberensis by Orthogonal Design

作　　者：黄和平[1,2] 高山林[3] 黄璐琦[2] 黄鹏[1] 汪电雷[1] 

机构地区：[1]安徽中医学院,安徽合肥230031 [2]中国中医科学院,北京100700 [3]中国药科大学,江苏南京210009

出　　处：《中药材》2013年第5期695-698,共4页Journal of Chinese Medicinal Materials

基　　金：国家科技重大专项(2009ZX09301-005-03-03);安徽省高等学校自然科学研究重点项目(KJ2011A191)

摘　　要：目的:优选盾叶薯蓣胚性愈伤诱导的最佳培养基组成。方法:采用茎尖分生组织进行离体培养获取盾叶薯蓣试管苗。切取试管苗不同部位诱导胚状体和愈伤组织,筛选最佳外植体;以生长率和薯蓣皂苷元含量作为指标,采用正交实验筛选胚性愈伤诱导培养基的最佳激素配比。结果:诱导愈伤组织和胚状体的最佳外植体为试管苗叶片,诱导率分别为92.5%和42.5%;胚性愈伤诱导最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+2,4-D 1.0 mg/L。结论:本研究结果可实现盾叶薯蓣胚性愈伤的有效诱导,为后续人工种子研究提供基础依据。Objective ： To select the suitable medium to induce embryogenic callus of Dioscorea zingiberensis. Methods ： Plantlet of Dioscorea zingiberesis in vitro was obtained by using apical meristem as explant. The different parts of the plantlets were cultured to se-lect the best explant used for inducing callus and embryoids. Growing rate and diosgenin content were calculated in orthogonal test to optimize combination of phytohormones for inducing embryogenic callus. Results： The leaves were suitable explants to induce callus and embryoid. The inducing rate of callus and embryoids reached 92.5% and 42.5%, respectively. The optimal medium for inducing embryogenic callus was MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.5 mg/L ＋ 2,4-D 1.0 mg/L. Conclusion ： The resuhs of this study can be used for effective induction of embryogenic callus of Dioscorea zingiberensis, and lay the foundation for the subsequent research of artifi-cial seeds.

关 键 词：盾叶薯蓣 胚性愈伤 正交实验 

分 类 号：R282.2[医药卫生—中药学]