HPLC定量指纹图谱法评价川麦冬质量研究  

Development of Full-Quantified HPLC Fingerprint for Quality Evaluation of Ophiopogonis Radix of Sichuan

作　　者：王进[1] 刘汉清[1] 刘霖[1] 唐海涛[2] 张平[2] 唐云[1] 

机构地区：[1]南京中医药大学,江苏南京210046 [2]江苏苏中药业集团股份有限公司,江苏泰州225500

出　　处：《中药材》2013年第5期721-725,共5页Journal of Chinese Medicinal Materials

基　　金：江苏省重点科研成果产业化项目(BA2011124)

摘　　要：目的:建立川麦冬药材的HPLC指纹图谱,并同时测定2种高异黄酮(甲基麦冬二氢高异黄酮A、甲基麦冬二氢高异黄酮B)的含量。方法:采用HPLC化学定量指纹图谱的方法,建立生脉注射液原料道地药材川麦冬的HPLC指纹图谱和活性成分的含量测定方法。色谱条件为Waters symmetry shieldTMRP 18(4.6 mm×250 mm,5μm)色谱柱,symmetry shieldTMRP 18预柱,以乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱,流速:1.0 mL/min,检测波长:280 nm,柱温:30℃。结果:得到了分离度、重现性较好的川麦冬药材HPLC指纹图谱,共标定了24个共有峰,各批次药材的相似度在0.98以上;通过对照品比对,确定了第14号峰及第15号峰分别为甲基麦冬二氢高异黄酮A、甲基麦冬二氢高异黄酮B,并对其定量分析。结论:所建立的HPLC定量指纹图谱灵敏度高、专属性强,可作为生脉等注射液麦冬原药材的质量控制依据。Objective： To establish HPLC fingerprint of Ophiopogonis Radix of Sichuan and simuhaneously determine two ho- moisoflavonoids （ methylophiopogonanones A and B ）. Methods：Full-quantified HPLC fingerprint was used to establish the HPLC fin-gerprint and determine the active ingredients of the daodi medicinal material Ophiopogonis Radix of Sichuan in Shengmai injection. Chromatographic condition was as follows： The analytical column was Waters symmetry shieldTM RP 18 （4.6 mm x 250 ram, 5 μm） with a pre-column of symmetry shieldTM RP 18. The mobile phase was acetonitrile-water （containing 0.1% phosphoric acid） with gra-dient elution. The flow rate was 1.0 mL/min, the detection wavelength was set at 280 nm and the column temperature was maintained at 30 ℃. Results： The HPLC fingerprint of Ophiopogonis Radix of Sichuan was established with good separation and repeatability. 24 common peaks were defined in the HPLC fingerprint. The similarity among batches was more than 0.98. Compared with standard refer-ence substances, No. 14 peak was methylophiopogonanone A and No. 15 peak was methylophiopogonanone B. Similarity determine sys-tem was applied to evaluate them. Conclusion： This analytical method is highly sensitive with strong specificity, which can be used ef-ficiently in the quality control of Ophiopogonis Radix of Sichuan in Shengmai injection.

关 键 词：川麦冬 定量指纹图谱 甲基麦冬二氢高异黄酮A 甲基麦冬二氢高异黄酮B 

分 类 号：R282.5[医药卫生—中药学]