不同品种不同产地大黄UPLC指纹图谱研究  

UPLC Fingerprint of Rhei Radix et Rhizoma from Different Habitats

作　　者：杜清涛[1] 温金莲[1] 严优芍[1] 郭丽冰[1] 

机构地区：[1]广东药学院,广东广州510006

出　　处：《中药材》2013年第5期725-731,共7页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81073045)

摘　　要：目的:建立不同产地大黄饮片和对照药材的UPLC指纹图谱,研究3个品种大黄的区别特征和大黄共有成分,比较不同分析方法的应用。方法:采用超高效液相色谱法(UPLC),ACQUITY BEH C18柱,0.1%磷酸溶液(A)-乙腈(B)梯度洗脱,检测波长260 nm,流速0.21 mL/min,柱温30℃。指纹图谱采用相似度分析、聚类分析和主成分分析。结果:3个品种大黄对照药材区别特征明显,不同产地大黄共有峰19个,经对照品对比及液质分析,确定了其中14个峰的成分。通过聚类分析,21批市售大黄样品被聚为5大类,与品种没有直接相关性,与相似度分析结果一致。对大黄药材饮片的质量评价,需要结合主成分分析提示的4个较重要成分的含量及样品整体图谱的分析,相似度分析和聚类分析对多基源药材的质量评价有一定局限性。结论:该方法分离度好、节省时间和溶剂,重现性好,可用于3个品种大黄对照药材的区别,不能用于市售大黄药材饮片的品种鉴定。Objective ： To establish the UPLC fingerprint of Rhei Radix et Rhizoma and reference crude drugs, analyze the charac-teristics among fingerprints of three species of reference crude drugs and the common components of Rhei Radix et Rhizoma, and com-pare the application of different analysis methods. Methods： UPLC procedure was performed on ACQUITY BEH C18 chromatographic column with mobile phase consisted of water （contained 0.1% phosphoric acid）-aeetonitrile （gradient elution） at a flow rate of 0.21 mL/rain. Detection wavelength was set at 260 nm and the column temperature was set at 30 ℃. Fingerprints were analyzed by similar-ity evaluation, cluster analysis and principal component analysis. Results： There were obvious characteristics among fingerprints of three species of reference crude drugs, 19 common chromatographic peaks were obtained from Rhei Radix et Rhizoma and 14 peaks were identified according to standard reference substances and by HPLC-MS. The cluster analysis and similarity evaluation showed the same result that 21 batches of sample were grouped into 5 categories and the result had no direct correlation with the botanical species. Both the contents of 4 important ingredients suggested by principal component analysis and the whole fingerprint analysis were necessary in quality evaluation of Rhei Radix et Rhizoma. There was certain limitation in quality evaluation of multiple sources drug which analy-sis by similarity evaluation and cluster analysis. Conclusion： The method with good reproducibility and separation saves time and sol-vent, it can he used in identification of three species of reference crude drugs but can not be used in species identification of commer-cial Rhei Radix et Rhizoma.

关 键 词：大黄 指纹图谱 相似度分析 聚类分析 主成分分析 

分 类 号：R282.5[医药卫生—中药学]