川楝子对神经系统的作用及机理探讨  

Effect of Meliae Toosendan Fructus on Nerves System and Its Mechanism

作　　者：向晓雪[1] 唐大轩[1] 熊静悦[1] 梁娅君[1] 牟道华[1] 谭正怀[1] 

机构地区：[1]四川省中医药科学院,四川成都610041

出　　处：《中药材》2013年第5期767-771,共5页Journal of Chinese Medicinal Materials

基　　金：国家"973"确有疗效的有毒中药科学应用关键问题的基础研究(2009CB522801)

摘　　要：目的:探讨炒黄川楝子乙醇提取物(川楝子提取物)对神经系统的作用机理。方法:采用热板法、辐射热刺激法及电刺激法观察川楝子提取物对小鼠感觉神经的影响,并测算小鼠热痛阈值,A-δ纤维、C纤维的兴奋幅度值,易化幅度值;运用在体间接检测法,考察川楝子提取物对大鼠坐骨神经的神经传导速度(NCV)的影响,通过电镜检测及银染色技术观察川楝子提取物对坐骨神经的病理学改变。结果:川楝子提取物可提高小鼠的热痛阈值及尾痛阈值,延缓大鼠NCV。电镜检查结果表明川楝子提取物可导致大鼠坐骨神经髓鞘纤维脱髓鞘,雪旺氏细胞数目减少。银染色雪旺氏细胞统计结果表明川楝子提取物可显著减少大鼠坐骨神经雪旺氏细胞的数目。结论:川楝子提取物能降低小鼠的痛觉敏感性,延缓大鼠NCV,其机制可能与减少雪旺氏细胞的数目有关。Objective：To study the effect of the ethanol extract of stir-bake to yellowish Meliae Toosendan Fructus on nerve system and its mechanism. Methods：The effect of the ethanol extract on sensory nerve was carried out through ache models induced by hot board method and radiant heat stimulation method in mice. The thermalgesia liminal value was investigated. The effect of the ethanol extract on the A-δ fiber and C fiber was measured by electrical stimulation procedure. Motor nerve conduction velocity （ NCV ） was measured by indirect detection method in vivo. The pathology changes of the motor nerve were observed by transmission electron micro- scope and the silver stain test. Results ：The ethanol extract of Meliae Toosendan Fructus could increase the thermalgesia liminal value of mice and reduce the conduction velocity of motor nerves. Meanwhile, pathology results showed the changes of the fiber of motor nerve,including demyelination and the number of Schwann cells dropping. Conclusion ： The ethanol extract of stir-bake to yellowish Me-liae Toosendan Fructus can reduce the pain sensitivity of mice and slow down NCV, which may be related to decreasing of the number of Schwann cells.

关 键 词：川楝子提取物 神经系统 痛阈值 NCV 病理学改变 

分 类 号：R285.5[医药卫生—中药学]