秃疮花中异紫堇碱的提取分离纯化工艺研究  

Extraction and Purification of Isocorydine from Dicranostigma leptopodum

作　　者：冯艳[1,2] 杨爱梅[1] 柳军玺[2] 邸多隆[2] 刘言娟[2] 李敏[1,2] 

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050 [2]中国科学院兰州化学物理研究所/中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物研究重点实验室,甘肃兰州730000

出　　处：《中药材》2013年第5期807-809,共3页Journal of Chinese Medicinal Materials

基　　金：甘肃省科技支撑计划(090NKCA127);中科院"西部之光联合学者"项目资助

摘　　要：目的:研究秃疮花中提取异紫堇碱的最佳工艺条件及采用大孔吸附树脂分离纯化异紫堇碱的方法。方法:采用正交试验设计,优化异紫堇碱的提取工艺;通过静态吸附/解吸附试验筛选最佳吸附异紫堇碱的大孔树脂。结果:最佳提取条件为:料液比1∶15(g/mL)、1%的酸水溶液为溶剂,提取时间1h×3次;LX28树脂为最佳吸附树脂。结论:采用最佳试验条件提取秃疮花,异紫堇碱得率可达0.88%;提取液经LX28树脂分离纯化,得到质量分数为85.34%的异紫堇碱,产率达68.64%。Objective：To optimize the conditions of extraction and purification of isocorydine from Dicranostigma leptopodum. Methods ： Extraction conditions of isocorydine were selected on the basis of orthogonal experimental design, the static adsorptiona/desorp-tion experiments were used to evaluate the optimum resin. Results ： The optimum extraction parameters were as follows ： the ratio of raw materials to solvent 1：15 （g/mL）, extraction solvent 1% vitriol, the extraction three times and 1 h each time. LX28 resin exhibited higher adsorption efficiency. Conclusion：Under the above optimum conditions,the extraction yield of isocorydine is 0.88%. The purity of isocorydine can reach 85.34% with a yield rate of 68.64%.

关 键 词：异紫堇碱 提取工艺 正交试验 大孔树脂 分离纯化 

分 类 号：R284.2[医药卫生—中药学]