中药仙茅不同有效成分对骨髓间质干细胞诱导作用的比较  

Comparison of different effective ingredients of Rhizoma curculiginis in bone marrow mesenchymal stem cells indued

作　　者：沈骅睿[1] 扶世杰[1] 汪国友[1] 李光胜[2] 徐祖健[1] 陈贵全[1] 

机构地区：[1]泸州医学院附属中医医院骨科,646000 [2]广安市人民医院骨科

出　　处：《中国实用医药》2013年第21期12-13,共2页China Practical Medicine

摘　　要：目的比较中药仙茅中不同有效成份对骨髓间质干细胞诱导作用的差异。方法分别采用RT-PCR检测和倒置显微镜下观察的方法 ,对使用中药仙茅多糖和仙茅黄酮诱导刺激后的骨髓间质干细胞进行检测,观察仙茅不同有效成分对骨髓间质干细胞刺激后的变化。结果仙茅多糖和仙茅黄酮成分均能诱导骨髓间质干细胞向神经元细胞诱导分化。结论仙茅黄酮较仙茅多糖对骨髓间质干细胞向神经元细胞定向诱导分化的作用更强。Objective Comparison of Chinese medicine Rhizoma curculiginis effective ingredients in different difference of bone marrow mesenchymal stem cells induced by using the method of observation;Method RT-PCR detection and inverted microscope respectively,to detect the polysaccharides and flavonoids from Curculigo orchioides stimulated using traditional Chinese medicine Rhizoma curculiginis after bone marrow mesenchymal stem cells,different observation of Curculigo orchioides the effective components of bone marrow mesenchymal stem cells after stimulation.Results Curculigo polysaccharide and Rhizoma curculiginis flavonoids could induce bone marrow mesenchymal stem cells differentiate into neurons.Conclusion Curculigo flavonoids is stronger Curculigo polysaccharide on bone marrow mesenchymal stem cells differentiation into neuron cell orientation.

关 键 词：仙茅多糖 仙茅黄酮 骨髓间质干细胞 神经元细胞 神经胶质细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]