产漆酶裂褶菌的筛选及其产酶培养条件优化研究  

Isolation of Schizophyllum commune Producing Laccase and Optimization of Laccase Production Conditions

作　　者：赵文娟[1] 徐升运[1] 任平[1] 

机构地区：[1]陕西省科学院酶工程研究所/陕西省酶工程技术中心,西安710600

出　　处：《湖北农业科学》2013年第12期2792-2797,共6页Hubei Agricultural Sciences

基　　金：陕西省科学院计划项目(2011K-11)

摘　　要：从采自秦岭的森林腐木菌苔上分离到一株产漆酶的裂褶菌(Schizophyllum commune Fr.)菌株mys005,对其产漆酶培养方式、培养基组成及培养条件进行了优化试验。结果表明,裂褶菌菌株mys005的产漆酶适宜培养方式为液体浅层振荡培养,含有Cu2+、Mn2+、Zn2+的微量元素复合液、洋葱(Allium cepa L.)、麦(Triticum aestivum L.)麸对其产漆酶都有较大的促进作用,而愈创木酚则对其生长和产漆酶有抑制作用。由新鲜马铃薯(Solanum tuberosum L.)200 g、K2HPO4 1.00 g、NaCl 0.50 g、MgSO4.7H2O 0.50 g、NaNO32.50 g、CaCl2.2H2O 0.10 g、FeCl30.02 g、吐温-80 1.20 mL溶于1 000 mL去离子水中组成的培养基在添加微量元素复合液(50 mg/mL CuCl2.2H2O+20 mg/mL MnSO4.H2O+10 mg/mL ZnSO4.7H2O)3 mL、洋葱100 g、麦麸10 g后,并且培养基起始pH 4.5、培养温度30℃、培养摇床转速200 r/min、培养时间7d,则摇瓶发酵的漆酶产酶量最高可达1 121 U/mL,显示出了良好的工业开发潜力。A Schizophyllum commune Fr.strain mys005 producing laccase was isolated from the rotten wood lawn in forest of Qinlin.The culture method for laccase production,medium composition and cultivation conditions was optimized.Results showed that the suitable cultivation method for S.commune strain mys005 to produce laccase was shallow liquid shaking culture.Trace elements compound liquid containing Cu2＋,Mn2＋,Zn2＋,onion（Allium cepa L.）,wheat（Triticum aestivum L.） bran could promote laccase production;while guaiacol could inhibit the growth and laccase production.If adding trace element compound solution（50 mg / mL CuCl2.2H2O＋20 mg / mL MnSO4.H2O＋ 10 mg / mL ZnSO4.7H2O） 3 mL,onion 100 g,wheat bran 10 g,in the medium containing fresh potato（Solanum tuberosum L.） 200 g,K2HPO4 1.00 g,NaCl 0.50 g,MgSO4.7H2O 0.50 g,NaNO3 2.50 g,CaCl2.2H2O 0.10 g、FeCl3 0.02 g、tween-80 1.20 mL in 1 000 mL deionized water,and keeping the starting pH at 4.5,starting temperature at 30 ℃,culture shaker speed at 200 r / min,culturing for 7 d,the laccase enzyme production yield in shake flask fermentation could be up to a maximum of 1 121 U / mL,showing great potential for industrial development.

关 键 词：漆酶 裂褶菌(Schizophyllum communeFr ) 筛选 产酶培养基 优化 

分 类 号：Q554.9[生物学—生物化学] S646.19[农业科学—蔬菜学]