新型生物反应器用于构建组织工程血管的初步探索  

Preliminary exploration of a new type of bioreactor for constructing tissue engineered blood vessels

作　　者：周嘉辉[1,2] 吴岳恒[1,2] 李东风[3] 郑坚奕[4] 林展翼[3] 

机构地区：[1]广东省人民医院(广东省医学科学院) [2]广东省心血管病研究所心内科 [3]广东省人民医院(广东省医学科学院)广东省老年医学研究所,广州510080 [4]南方医科大学

出　　处：《广东医学》2013年第13期1971-1973,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30901468);广东省科技计划项目(编号:2009B060700116);广东省自然科学基金资助项目(编号:10151008008000011)

摘　　要：目的设计一种实用的生物反应器,并探讨把它应用于组织工程血管(TEBV)培养的可行性。方法根据国外文献所报道的基本原理,设计一种实用的生物反应器,把人脐动脉血管平滑肌细胞(VSMCs)接种到管状聚乙醇酸(PGA)无纺布培养支架上,放入生物反应器内进行三维压力培养,2周后收获培养新生物,肉眼观察大体外观并拍照记录,扫描电镜检测表面形貌。结果 VSMCs在生物反应器内经2周连续培养初步形成一条血管样组织。新生组织外观光滑平整,色泽鲜亮,具有一定厚度和弹性,能自我维持管腔形态。扫描电镜显示组织含有丰富的细胞外基质(ECM),PGA无纺布已被ECM完全包裹,管壁有未降解的PGA碎片。结论设计的生物反应器符合组织工程培养的要求,未来可以用其进行构建TEBV的进一步研究。Objective To study the application of a new practical bioreactor in tissue engineered blood vessels（TEBV） cultured.Methods A new bioreactor was designed according to the basic principles reported in literatures.Human umbilical artery smooth muscle cells（VSMCs） were seeded on the scaffold of polyglycolic acidr 2 weeks for electron microscopy scanning.Results A vessel-like tissue was constructed during the 2-week culture of VSMCs in the closed bioreactor.The constructed vessel bright and smooth in appearance,with certain thickness and flexibility,and self-maintenance of lumen shape.According to electron microscopy,rich extracellular matrix was observed in the constructed vessel,which distributed evenly and wrapped the PGA completely.There were incompletely degraded PGA residues among the tube wall.Conclusion The bioreactor meets the requirements of TEBV construction,and it could be used for further research.

关 键 词：生物反应器 组织工程 血管 压力 三维培养 

分 类 号：R318.11[医药卫生—生物医学工程]