保健酒违法添加奥沙普嗪免疫学检测方法建立  

Development of an Immunoassay for Detection of Oxaprozin as Illegal Adulterant in Health Liquor

作　　者：郭杰标[1] 李杏娉[1] 郝卿辰[1] 钟瑞敏[1] 何颖娟[1] 刘艳珊[1] 

机构地区：[1]韶关学院,广东韶关512005

出　　处：《食品研究与开发》2013年第11期48-50,86,共4页Food Research and Development

基　　金：香港铭源基金科研项目(2010/188);广东省营养膳食与健康重点实验室开放基金项目(2011K004);广东省自然科学基金项目(S2011040001362)

摘　　要：建立快速检测违法添加剂奥沙普嗪的免疫学方法。以活泼酯法将奥沙普嗪分别连接到BSA和OVA上制备人工抗原,免疫家兔制备多克隆抗体,经条件优化建立竞争抑制酶联免疫吸附检测方法。结果显示:检测体系IC50值为3.1 ng/mL,最低检测限为0.5 ng/mL,与8种相关药物交叉反应率都小于0.1%。用ELISA方法与HPLC检测30种市售保健酒,1例阳性和29例阴性结果全部吻合。To develop an immunoassay for detection of oxaprozin, oxaprozin was conjugated to BSA and OVA to synthesize artificial antigens using active ester method. Polyclonal antibodies were obtained from immunized rabbits. The IC50 value and LOD of the optimal ELISA were 3.1 ng/mL and 0.5 ng/mL, respectively. All cross reactivities against 8 associate drugs were less than 0.1%. The ELISA was in good agreement with LC-UV when detected oxaprozin in 1 positive and 29 negative health liquors from market.

关 键 词：功能食品 奥沙普嗪 违法添加 人工抗原 免疫检测 

分 类 号：R155.5[医药卫生—营养与食品卫生学]