血管紧张素转移酶基因插入/缺失多态性与儿童哮喘的关系  

DD genotype of angiotensin-converting enzyme may be a risk factor for development of asthma in children

作　　者：秦建华[1] 王鸾升[1] 

机构地区：[1]大连医科大学附属第一医院儿科,116011

出　　处：《中华儿科杂志》2000年第8期487-489,共3页Chinese Journal of Pediatrics

摘　　要：目的　探讨血管紧张素转移酶 (ACE)基因插入 /缺失多态性与儿童哮喘易感性的关系。方法　应用聚合酶链反应方法检测 5 2例哮喘患儿 ,5 4例肺炎患儿及 40例正常儿童的ACE基因型。用呼气峰速仪测定 2 3例哮喘患儿的最大呼气流量作为评价肺功能的指标。结果　三组儿童ACE基因型 (II型、ID型、DD型 )频率的分布差异有显著意义 (P <0 .0 5 )。哮喘组DD基因型频率为 34.6 % ,与正常组 ( 12 .5 % )和肺炎组 ( 14.8% )比较 ,差异有显著意义 (P均 <0 .0 5 )。哮喘组和正常组比较 ,DD型与ID型、DD型与II型的优势比OR分别为 5 .0 4和 3.15 ( 95 %可信区间 )。不同ACE基因型间比较哮喘患儿最大呼气流量占预计值的百分比 ,差异无显著意义 (P >0 .0 5 )。结论　ACE基因DD基因型与哮喘的易感性有关 ,可能是儿童哮喘的危险因素。Objective The insertion/deletion polymorphism of angiotensin-converting enzyme (ACE) gene in intron 16 of a 287 bp nonsense domain was identified and shown to be closely associated with the levels of ACE in plasma and cells. ACE is heavily expressed in the lung and plays a key role in the metabolism of angiotensin II and inactivation of bradykinin peptides and substance P, which are potent bronchial constrictors and inflammatory mediators of asthma. The present study was conducted to assess the relationship between the insertion/deletion polymorphism of ACE gene and asthma in children. Methods ACE genotypes were determined by polymerase chain reaction (PCR) in 52 asthmatic children, 54 children with pneumonia and 40 healthy controls. The peak expiratory flow rate (PEFR) was determined to evaluate the lung function of 23 asthmatic children. Results The ACE genotype (II, ID and DD) distribution was significantly different among the three groups (P<0.05). There was a high frequency of DD genotype of ACE gene in asthmatic children compared with the children with pneumonia and healthy controls (34.6% vs.14.8%, 34.6% vs.12.5%, P<0.05). The odds ratio (OR) for developing asthma between patients with DD genotype and those with ID genotype and II genotype was 5.04 and 3.15, respecively. The asthmatic children were classified into two groups (DD and non-DD) according to their genotypes to compare their PEFR ratio; the result did not show any significant difference (P>0.05).Conclusion DD genotype of ACE gene may be related to susceptibility of children to asthma and may be a risk factor in development of asthma.

关 键 词：哮喘 儿童 ACE基因 基因多态性 DD基因 

分 类 号：R725.6[医药卫生—儿科]