HBsAg健康携带者自然转阴后外周血淋巴细胞SCE频率的变化  

A CHANGE OF FREQUENCY OF SISTER CHROMATID IN PERIPHERAL LYMPHOCYTES OF HEALTHY CARRIERS WITH HBsAg AFTER TRANSFORMED TO HBsAg NEGATIVITY NATURALLY

作　　者：王振华[1] 王瑜[2] 苏爱[1] 王培林[1] 

机构地区：[1]青岛大学医学院生物学教研室,青岛266021 [2]青岛大学医学院第二附属医院小儿科,青岛266021

出　　处：《青岛大学医学院学报》2000年第1期31-32,共2页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：1目的　探讨 HBs Ag健康携带者自然转阴后外周血淋巴细胞姐妹染色单体互换 (SCE)频率的变化。 2方法　用常规法培养外周血淋巴细胞并用 5 -溴脱氧尿嘧啶核苷诱导 SCE,用紫外线照射法制备 SCE标本 ,对 2 0例 HBs Ag健康携带者 5年自然转阴后外周血淋巴细胞的 SCE频率进行检测 ,同时将 2 0例 HBs Ag阳性的健康携带者作为配对组 ,35例 HBs Ag阴性的健康人作为正常对照组进行 SCE频率的检测。 3结果　上述 3组间的SCE频率不同 (F =3.40 4,P<0 .0 5 ) ,配对组的 SCE频率明显高于对照组 ,差异有显著性 (q=3.6 98,P<0 .0 5 ) ,而转阴组与配对组、对照组相比差异均无显著性 (q=2 .0 17,1.42 3,P>0 .0 5 )。4结论　机体自身有对 HBV感染所致DNA损伤修复的能力。Objective To investigate the change of frequency of sister chromatid exchange (SCE) in the peripheral lymphocytes of healthy carriers with HBsAg after transformed to HBsAg negativity naturally. Methods The peripheral lymphocytes were cultured according to the routine methods. SCE of the peripheral lymphocytes was induced by BrdU and the SCE slides were prepared using the UPG method. The frequency of SCE of 20 healthy carriers with HBsAg after transformed negative in 5 years were observed. The frequencies of 20 healthy carriers with HBsAg positive as a pairing group and 35 normal control individuals were also observed. Results The frequencies of SCE of the three groups were different(F=3.404,P<0.05). The frequency of SCE of the pairing group was significantly higher than that of the control group (q=3.698, P<0.05), but there was no significant difference in SCE between the transformed negative group and pairing group or control group(q=2.017,1.423, P>0.05). Conclusion The human body may have the ability of repairing the DNA damage caused by the infectin of hepatitis B virus. [

关 键 词：姐妹染色单体交换 乙型肝炎 HBSAG SCE频率 

分 类 号：R392[医药卫生—免疫学]