缺血预处理对移植肝缺血再灌注损伤中细胞凋亡的影响  

Effects of ischemic preconditioning on apoptosis of hepatocytes in liver transplantation in rats

作　　者：张效杰[1] 曹晖[1] 焦哲[1] 凌伟[1] 吴志勇[1] 陈治平[1] 邝耀麟[1] 

机构地区：[1]上海第二医科大学仁济医院普外科

出　　处：《中华肝脏病杂志》2000年第4期221-223,共3页Chinese Journal of Hepatology

摘　　要：目的探讨细胞凋亡在移植肝缺血再灌注损伤中的作用及缺血预处理对其影响。方法通过对移植肝进行缺血预处理，用全自动生化分析仪检测肝功能、比色法测定移植肝组织的ＭＤＡ、用流式细胞仪结合原位末端标记技术检测细胞凋亡。结果移植肝再灌注后血中ＡＳＴ，ＡＬＴ，ＬＤＨ和肝组织中ＭＤＡ均明显升高，肝细胞凋亡明显增加，经缺血预处理后，血中ＡＳＴ，ＡＬＴ，ＬＤＨ和肝组织中ＭＤＡ均降低，肝细胞凋亡亦明显减少。结论缺血预处理可以通过减轻移植肝脂质过氧化反应，从而减少肝细胞凋亡，使肝细胞损伤减轻从而保护移植肝。Objective To evaluate the effects of ischemic preconditioning (IP) on hepatocyte apoptosis in ischemia/reperfusion injury of grafted liver and clarify its mechanism. Methods Orthotopic liver transplantation with cuff anastomosing method for the portal vein, suprahepatic vena cava and infrahepatic vena cava in the rats were divided into two groups: with and without IP treatment. The parameters of liver function were determined by auto-biochemical analyser. The concentrations of malondialdehyde (MDA) were measured with chromometry. The apoptotic hepatocytes in grafts liver were detected by flow cytometry combined with the in situ terminal deoxynucleotidyl transferase-mediated dUTP-FITC nick endlabeling (TUNEL) method. Results The levels of AST, ALT, LDH in the serum, and MDA and apoptotic cells in the liver were significantly increased compared with normal values after ischemia/reperfusion to the grafted liver. After IP treatment, the levels of AST, ALT, LDH, MDA and apoptotic cells were significantly decreased. Conclusion IP can attenuate ischemia/reperfusion injury by alleviating lipid peroxide and decreasing apoptosis of hepatocytes.

关 键 词：缺血预处理 肝移植 再灌注损伤 细胞凋亡 

分 类 号：R657.3[医药卫生—外科学]