c-myc、p53和bcl-2与银屑病角质形成细胞凋亡异常关系的研究  

Study on the Relationship Between c-myc, p53 and bcl-2 and Abnormal Apoptosis of Keratinocytes in Psoriatic Lesions

作　　者：倪晓[1,2] 孙建方(指导)[2] 曾学思[2] 桑红桂[2] 李阿梅[2] 叶干运(指导)[2] 

机构地区：[1]南京市鼓楼医院 [2]中国医学科学院,中国协和医科大学皮肤病研究所,南京210042

出　　处：《中华皮肤科杂志》2000年第4期240-242,共3页Chinese Journal of Dermatology

基　　金：卫生部基金资助课题

摘　　要：目的 探讨原癌基因 c-myc、抑癌基因 p53和凋亡保护基因 bcl-2在银屑病角质形成细胞凋亡异常中的作用。方法 采用免疫组化和非同位素核酸原位杂交技术从蛋白和 mRNA二个水平检测了 36份寻常型银屑病皮损中 c-myc、 p53和 bcl-2的表达状况。结果 银屑病表皮中的 c-myc、 p53和增殖细胞核抗原（ PCNA）阳性细胞较正常皮肤明显增加，而 bcl-2阳性细胞在表皮基底层中明显减少。在 PCNA阳性细胞 >30的银屑病皮损中，有更多的 c-myc阳性细胞Objective To investigate the effects of proto-oncogene c-myc, tumor suppressor gene p53 and apoptosis protection gene bcl-2 on abnormal apoptosis of keratinocytes in psoriatic lesions. Methods Expression of c-myc, p53, bcl-2 was analysed by both immunohistochemical methods and nucleic acid in situ hybridization in a total of thirty six lesional skin samples from patients with psoriasis vulgaris. Results c-myc, p53 and PCNA-positive keratinocytes increased significantly in psoriatic lesions in comparison with the controls,especially in the lower and basal layers of epidermis. bcl-2-positive cells decreased in basal layers of psoriatic skin. In lesions with more than 30 PCNA-positive cells, there were a significant increase of c-myc protein (P

关 键 词：p53基因 C-MYC基因 BCL-2基因 银屑病 

分 类 号：R758.6[医药卫生—皮肤病学与性病学]