Study on Characters of Major Hydrolase Isozymes in Wheat Seeds  

小麦种子中主要水解酶同工酶特性研究(英文)

作　　者：李延红[1] 韩卓[1] 陈晓燕[1] 孙汉巨[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《Agricultural Science & Technology》2013年第7期959-961,972,共4页农业科学与技术（英文版）

基　　金：Supported by the 2010 Yangtze River Delta Joint Key Project of Anhui Province(10140702023);2012 Collegiate Innovation Training Program for the Students of Hefei University of Technology(2012CXCY394);National Natural Sciences Foundation of China(31171787);2010 Provincial Scientific Research Program of Anhui Higher Education Institutions(010AJZR0899)~~

摘　　要：ObjectiveThis study aimed to investigate the characters of major hydrolase isozymes in wheat seeds. MethodThe spectra of amylase, esterase and proteolytic enzyme isozymes in the germ, endosperm and radicle were studied in detail during the germination process using the polyacrylamide gel electrophoresis（PAGE）. ResultThe results indicated that isozymes of amylase and esterase changed obviously in the endosperm during the germination, while isozymes of proteolytic enzyme exhibited distinct variation in the germ and radicle. ConclusionThis study provides basic data for effeciently improving the germination rate of crop seeds in the future.[目的]研究小麦种子中主要水解酶同工酶的特性。[方法]采用聚丙烯酰胺凝胶(PAGE)电泳技术,分别对萌发过程中小麦种子中胚芽、胚乳和胚根三大组织部位存在的淀粉酶、酯酶、蛋白水解酶的同工酶图谱进行了研究,[结果]实验结果表明,在小麦种子胚乳中的淀粉酶、酯酶同工酶变化较明显,而蛋白水解酶变化较大的则是在胚芽和胚根当中。[结论]该研究为今后有效提高作物种子的萌发率提供了基础资料。

关 键 词：Wheat GERMINATION AMYLASE ESTERASE PROTEASE 

分 类 号：S512.1[农业科学—作物学]