兔骨折模型应用胎盘多肽的实验研究  

Study on placenta polypeptide injection treatment for fractures in a rabbit model

作　　者：周丕琪[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中医科,武汉430022

出　　处：《海南医学院学报》2013年第10期1333-1337,1341,共6页Journal of Hainan Medical University

基　　金：中国高校医学期刊临床专项资金项目(112210807)~~

摘　　要：目的:观察胎盘多肽注射液对家兔骨折模型愈合过程的影响。方法:选用30只雄性新西兰家兔,随机分为3组。正常组(10只)不予任何处理,模型组(10只)和治疗组(10只)均手术处理,制作骨折模型,治疗组家兔术后第6天开始每天注射胎盘多肽注射液4mL,所有家兔于4周后处死,观察骨折部位骨痂生长、骨生物力学、骨形态发生蛋白-2(BMP-2)、转化生长因子-β2(TGF-β2)、骨钙素(BGP)及血管内皮生长因子(VEGF)表达。结果:治疗组和模型组均有骨痂组织生长,治疗组及模型组中BMP-2、TGF-β2、BGP、VEGF表达量均高于正常对照组,且治疗组VEGF、BMP-2切片阳性细胞数高于模型组,但治疗组与模型组TGF-β2、BGP阳性细胞数大体相当。结论:胎盘多肽注射液在骨折愈合过程中促进骨痂组织的生长,可能与提高BMP-2、VEGF表达有关。Objective：To observe the effect of placenta polypeptide injection treatment on healing process of fractures in a rabbit model.Methods：Thirty male New Zealand rabbits were randomly divided into 3groups,rabbits in normal group（n=10）were without any treatment,and rabbit fracture model（model group,n=10）and treatment group（n=10）were established by surgery.Rabbits in treatment group were treated with 4mL placenta polypeptide injection 6days after surgery and all rabbits were sacrificed after 4weeks,then investigated the level of serum calcium,phosphonium and bone alkaline phosphatase,as well as bony callus growth,bone biochemistry and the expression of bone glaprotein（BGP）,bone morphogenetic protein（BMP-2）,transforming growth factorβ 2（TGFβ 2） and vascular endothelial growth factor（VEGF）.Results ： Both model group and treatment group showed bony callus growth,the expression of BMP-2,TFGβ 2,BGP and VEGF were significantly higher in model group and treatment group than in normal group.There were more VEGF and BMP-2positive cells in treatment group than that in model group,but the number of TFGβ 2and BGP positive cells were similar in treatment group than model group.Conclusion ： Placenta polypeptide injection promotes bony callus tissue growth during the fracture healing process,possibly due to the up-regulation of BMP-2and VEGF expression.

关 键 词：胎盘多肽注射液 骨折愈合 骨痂 

分 类 号：R68[医药卫生—骨科学]