Effects of Neutral Detergent Soluble Fiber and Sucrose Supplementation on Ruminal Fermentation, Microbial Synthesis, and Populations of Ruminal Cellulolytic Bacteria Using the Rumen Simulation Technique (RUSITEC)  

作　　者：ZHAO Xiang-hui LIU Chan-juan LI Chao-yun YAO Jun-hu 

机构地区：[1]College of Animal Science and Technology,Northwest A&F University

出　　处：《Journal of Integrative Agriculture》2013年第8期1471-1480,共10页农业科学学报（英文版）

基　　金：supported by the National Key Technologies R&D Program of China(2012BAD12B02);the Program of International S&T Cooperation of China(2010DFB34230);the Scientific&Technological Innovation Project of Shaanxi,China(2011KTCQ02-02)

摘　　要：We evaluated the effects of neutral detergent soluble fiber （NDSF） and sucrose supplementation on ruminal fermentation, microbial synthesis, and populations of ruminal cellulolytic bacteria using the rumen simulation technique （RUSITEC）. The experiment had a 2x2 factorial design with two dosages of sucrose, low （ca. 0.26 g d-1, low-sucrose） and high （ca. 1.01 g d-1, high-sucrose）, and two dosages of supplied NDSF, low （1.95 g d-1, Iow-NDSF） and high （2.70 g d-1, high-NDSF）. Interactions between NDSF and sucrose were detected for xylanase activity from solid fraction and apparent disappearance of neutral detergent fiber （NDF） and hemicellulose, with the lowest values observed for high-NDSF and high-sucrose treatment. Supplemental NDSF appeared to increase the molar proportion of acetate and reduce that of butyrate; however, the effects of supplemental sucrose on VFA profiles depended upon NDSF amount. There was a NDSFxsucrose interaction for the production of methane. High-NDSF fermenters had lower ammonia-N production, greater daily N flow of solid- associated microbial pellets and total microorganisms, and greater microbial synthesis efficiency compared with low- NDSF fermenters. Supplementation with NDSF resulted in an increase in 16S rDNA copies of Ruminococcusflavefaciens and a reduction in copies of Ruminococcus albus. Supplementation with sucrose tended to increase the 16S rDNA copies ofR. albus from liquid fraction, but did not affect daily total microbial N flow and cellulolytic bacterium populations from solid fraction. These data indicate that the effects of the interaction between NDSF and sugars on ruminal fermentation and fiber digestion should be taken into account in diet formulation. Ruminal fermentation and metabolism of sugars warrant further investigation.We evaluated the effects of neutral detergent soluble fiber （NDSF） and sucrose supplementation on ruminal fermentation, microbial synthesis, and populations of ruminal cellulolytic bacteria using the rumen simulation technique （RUSITEC）. The experiment had a 2x2 factorial design with two dosages of sucrose, low （ca. 0.26 g d-1, low-sucrose） and high （ca. 1.01 g d-1, high-sucrose）, and two dosages of supplied NDSF, low （1.95 g d-1, Iow-NDSF） and high （2.70 g d-1, high-NDSF）. Interactions between NDSF and sucrose were detected for xylanase activity from solid fraction and apparent disappearance of neutral detergent fiber （NDF） and hemicellulose, with the lowest values observed for high-NDSF and high-sucrose treatment. Supplemental NDSF appeared to increase the molar proportion of acetate and reduce that of butyrate; however, the effects of supplemental sucrose on VFA profiles depended upon NDSF amount. There was a NDSFxsucrose interaction for the production of methane. High-NDSF fermenters had lower ammonia-N production, greater daily N flow of solid- associated microbial pellets and total microorganisms, and greater microbial synthesis efficiency compared with low- NDSF fermenters. Supplementation with NDSF resulted in an increase in 16S rDNA copies of Ruminococcusflavefaciens and a reduction in copies of Ruminococcus albus. Supplementation with sucrose tended to increase the 16S rDNA copies ofR. albus from liquid fraction, but did not affect daily total microbial N flow and cellulolytic bacterium populations from solid fraction. These data indicate that the effects of the interaction between NDSF and sugars on ruminal fermentation and fiber digestion should be taken into account in diet formulation. Ruminal fermentation and metabolism of sugars warrant further investigation.

关 键 词：neutral detergent soluble fiber SUCROSE ruminal fermentation RUSITEC 

分 类 号：S816[农业科学—饲料科学]