去分化间充质干细胞成骨再分化潜能的实验研究  

Osteogenic redifferentiation of de-differentiated MSCs

作　　者：张慧[1] 王贵超[1] 韩兴龙[1] 顾巧丽[1] 黄晨[1] 谢芳[2] 施勤[1] 

机构地区：[1]苏州大学附属第一医院骨科,江苏苏州215006 [2]苏州大学基础医学与生物科学学院病理系,江苏苏州215021

出　　处：《南京医科大学学报（自然科学版）》2013年第8期1044-1048,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81101369);留学回国人员基金

摘　　要：目的:研究去分化间充质干细胞(de-differentiated mesenchymal stem cells,De-MSCs)成骨再分化能力。方法:以MSCs为对照,分别采用Cell Count Kit(CCK8)法、实时定量PCR(qPCR)、碱性磷酸酶(ALP)活性和茜素红染色法检测De-MSCs增殖能力、成骨相关基因、成骨再分化能力等。结果:De-MSCs可表达干细胞表面标志,在成骨培养基中增殖能力高于MSCs(P<0.05),成骨相关基因(Runx2、Osterix、Bmp2)表达明显增加(P<0.05),ALP活性检测发现De-MSCs明显高于MSCs组(P<0.05),28 d后茜素红染色可见红色结节。结论:De-MSCs具备某些MSCs特征,但是体外再次成骨效率明显提高,De-MSCs可作为更优质种子细胞,为再生医学开拓新的思路。Objective：To explore the osteogenic differentiation potential of de-differentiated mesenchymal stem cells（De-MSCs）.Methods：Compared with MSCs,the proliferation capacity and osteogenic related-gene expression of De-MSCs were detected using CCK8 and qPCR assay.Meanwhile the osteogenic redifferentiation potential was measured by the ALP activity assay and the bone formation was confirmed by alizarin red staining.Results：De-MSCs could express stem cells markers,proliferation capacity was obviously higher than MSCs in osteogenic medium statistically.The expression levels of osteogenic related genes and ALP activities were remarkblely increased compared with MSCs.Red nodules were observed with alizarin red staining after De-MSCs were cultured in osteogenic medium for 28 days.Conclusion：De-MSCs retain some MSC traits,with a higher osteogenic differentiation efficiency than MSCs.De-MSCs could be an alternative potential seed cells in tissue engineering.

关 键 词：去分化间充质干细胞 间充质干细胞 成骨再分化 

分 类 号：Q254[生物学—细胞生物学]