邻苯二甲醛衍生法测定工作场所空气中二乙烯三胺  

Determination of diethylenetriamine in workplace air with precolumn O-phthalaldehyde derivation

作　　者：刘汉方[1] 马德库[1] 梁戈清[1] 郭智斌[1] 

机构地区：[1]中国石油天然气集团公司石油职业卫生技术服务中心中心实验室,河北廊坊065000

出　　处：《中国卫生检验杂志》2013年第7期1665-1667,共3页Chinese Journal of Health Laboratory Technology

基　　金：2011年廊坊市科研课题(市级)项目(2011013022)

摘　　要：目的:建立碱性硅胶管采样,高效液相荧光检测器法测定工作场所空气中二乙烯三胺的方法。方法:使用碱性硅胶管采样,使用邻苯二甲醛作为衍生剂,激发波长351 nm,发射波长411 nm,高效液相荧光检测器法测定。结果:穿透容量大于4 mg,线性范围0.015μɡ/ml-10μɡ/ml,相关系数r=0.9995,变异系数小于3%。结论:本法测定工作场所空气中二乙烯三胺操作简便,结果可靠,值得推广。Objective： To establish a method for determination of diethylenetriamine in workplace air by alkaline silica gel tube sampling and high performance liquid chromatography（HPLC） with fluorescence detection. Methods： The diethylenetriamine in workplace air is collected by alkaline silica gel tube and derived with 0 --phthalaldehyde （OPA）, then the derivative sample was detected by fluorescence detector with excitation wavelength （kex） at 351 nm and emission wavelength （hem） at 411 nm. Results ： The calibration curve was linear within the range of 0. 015 μg/ml - 10 μg/ml. The correlation coefficient was 0. 9995. The extraction recoveries were 100.4% , with relative standard deviations of 3%. Conclusion： This method is simple, convenient, accurate and reproducible,which may be suitable for the determination of diethylenetriamine in the air of workplace.

关 键 词：碱性硅胶管 邻苯二甲醛 液相色谱 二乙烯三胺 

分 类 号：O657.72[理学—分析化学]