PCR法检测日本血吸虫初探  

Study on detection of Schistosoma japonicum by PCR

作　　者：刘勇[1] 周水茂[1] 杨光斌 范宏萍 唐丽 徐明星[1] 

机构地区：[1]武汉市疾病预防控制中心,湖北武汉430015 [2]湖北省血吸虫病防治研究所,湖北武汉430079

出　　处：《中国卫生检验杂志》2013年第7期1737-1738,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:初步探索PCR法检测日本血吸虫的效果。方法:日本血吸虫尾蚴1000条感染大白兔,6周后采血并收集大白兔肝脏,肝脏抽提虫卵,血清提取DNA,日本血吸虫非长末端重复序列反转录转座子SjR2基因序列设计引物,PCR检测日本血吸虫感染大白兔虫卵及血清中DNA。结果:8份日本血吸虫尾蚴感染大白兔虫卵和血清样品均扩增出230bp特征性条带。结论:PCR法检测日本血吸虫重度感染大白兔血清中DNA有较好效果。Objective：To explore the detection effect of Schistosoma japonicum by PCR. Methods： The blood and liver samples of white rabbits were collected six weeks after infected with 1000 Schistosoma japonicum cercariae. Eggs were extracted from liver. DNAs were extracted from serum samples. Specific primers were designed based on the non - long terminal repeat retrotransposon SjR2 gene sequence of Schistosoma japonicum, and then PCR assay was used to detect DNAs from samples of eggs and serum of rabbits infected with Schistosoma japonicum. Results： A 230 bp DNA characteristic fragment was obtained from 8 samples of eggs and serum of rabbits. Conclusion： Detec- tion of Schistosoma japonicum DNA from serum of rabbits with heavy infection by PCR has a better effect.

关 键 词：日本血吸虫 PCR 检测效果 

分 类 号：R446.5[医药卫生—诊断学]