去甲斑蝥素对G422脑胶质瘤抑制作用的体内研究  

Stydy on Inhibition of Gliomas Cell of NCTD in Vivo

作　　者：张青松[1] 李灿[1] 洪学军[1] 吴倩[1] 

机构地区：[1]襄阳市中心医院,湖北襄阳441021

出　　处：《中医药导报》2013年第7期80-82,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:研究去甲斑蝥素(NCTD)体内对脑胶质瘤G422细胞的抑制作用。方法:建立G422细胞小鼠移植瘤模型,将荷瘤小鼠随机分为模型组、CTX阳性对照组及NCTD高、中、低剂量组(100、50、25 mg.kg-1),每组10只,另设10只为正常对照组,正常对照组每日给生理盐水灌胃,CTX和NCTD组采用腹腔注射方式给药,10 d后双抗体夹心ABC-ELISA法测sIL-2R的含量;酶联免疫ELISA法测IFN-γ含量;剥瘤,计算抑瘤率;剥瘤的同时取脾脏,计算脾脏指数;MTT法检测NCTD对荷瘤小鼠脾脏T、B淋巴细胞增殖的影响。结果:NCTD可降低荷瘤小鼠血清sIL-2R含量,提高IFN-γ含量,抑制G422细胞小鼠移植瘤的生长;对脾脏指数影响较少,可促进荷瘤小鼠的T、B淋巴细胞增殖。结论:NCTD体内外可抑制G422细胞生长,并具有免疫调节作用。Objective： To study the inhibition of NCTD ongliomas in vivo.Methods： The mice with G422 cell xenografts were randomly divided into model group,positive control group,NCTD higher,middle and lower doses groups（100,50 and 25 mg.kg-1）,10 rats in each group.There is normal control group of 10 rats without inoculated tumour.After 10-day treatment,the serum content of sIL-2R was determined by enzyme-linked immunosorbent assay.The serum content of IFN-γ was detected by ELISA.Tumor was stripped and the tumor-inhibition rate was counted.The spleen was removed,with the spleen index calculated;MTT was performed to observe the effect of NCTD on proliferation of lymphocyte cell T and B.Results： NCTD significantly decreased the content of sIL-2R in mice,increased the content of IFN-γ,inhibited the growth of G422 cell xenografts,with no significant effect on spleen index and promoted the proliferation of lymphocyte T and B in tumor-bearing mice spleen.Conclusion： NCTD can inhibit the growth of G422 in vivo and promote immunomodulatory effects.

关 键 词：去甲斑蝥素 G422细胞 SIL-2R IFN-Γ 小鼠 

分 类 号：R285.5[医药卫生—中药学]