灵芝酸G对小鼠巨噬细胞免疫活性的研究  

Effect of ganoderic acid G chalcone-G on Macrophages of immunity activity in mice

作　　者：李仲娟[1,2] 杨朝令[1] 胡芳[2] 喻昕[1] 汪宏良[1,2] 

机构地区：[1]湖北理工学院医学院检验系,湖北黄石435003 [2]黄石市中心医院,湖北黄石435000

出　　处：《国际检验医学杂志》2013年第13期1640-1641,1644,共3页International Journal of Laboratory Medicine

基　　金：湖北省科技项目(编号2011Cdc116);黄石市科技项目(黄科农[2011]1号-16);湖北理工学院项目(编号:RCYJ20113012);湖北省重点学科湖北理工学院药学学科资助

摘　　要：目的研究灵芝酸G对小鼠巨噬细胞活化及肿瘤细胞的杀伤作用。方法流式细胞仪测定小鼠巨噬细胞的表面分子表达;ELISA法检测小鼠腹腔巨噬细胞分泌IL-1和TNF的水平;MTT法检测巨噬细胞因子对肿瘤细胞的抑制能力。结果灵芝酸G经过3天刺激能激活小鼠巨噬细胞表面分子CD68;体外实验显示,1μg/mL灵芝酸可以诱导小鼠巨噬细胞分泌IL-1和TNF-a;灵芝酸G诱导巨噬细胞因子抑制肿瘤细胞的能力与5μg/mL吉西他滨没有显著不同,但二者明显高于空白对照组。结论灵芝酸G能诱导小鼠巨噬细胞产生M1型细胞因子,杀伤肿瘤细胞。Objective To study the ganoderic acid Gon macrophage activation in mice and cell-killing effect on tumor cells.Me-thods Macrophages subface were measured with flow cytometry.IL-1and TNF-α in mice peritoneal macrophages were detected by ELISA.In hibition effect of macrophage factor on tumor cells was measured with MTTassay.Results After stimulation with ganoderic acid G,mice peritoneal macrophages in an increased expression of CD68 was up regulated and the IL-1 and TNF levels secreted by macrophages at 1 μg/mL with ganoderic acid G in vitro experiments.Compared with inhibition effect of tumor cells,there was no significant difference between ganoderic acid G induced macrophage cytokine and 5 μg/mL gemcitabine,but there was significant difference with the control group.Conclusion Type M1 macrophages in mice maybe is activated by ganoderic acid G,killing tumor cells.

关 键 词：灵芝 巨噬细胞 白细胞介素1 细胞因子类 流式细胞术 

分 类 号：R285.5[医药卫生—中药学]