54例轻链型单克隆免疫球蛋白血症的蛋白水平分析  

Analysis of protein level in 54 patients with light chain type of monoclonal gammopathy

作　　者：颜绵生[1] 王松子[1] 廖慧芳[1] 

机构地区：[1]中山大学附属第一医院检验科,广东广州510080

出　　处：《国际检验医学杂志》2013年第13期1649-1650,1655,共3页International Journal of Laboratory Medicine

摘　　要：目的研究分析54例轻链型单克隆免疫球蛋白血症的体液蛋白特征。方法利用免疫固定技术从2 765例单克隆免疫球蛋白血症中筛查出275例轻链型患者,对其中54例患者进行血清蛋白电泳、尿液本-周蛋白、尿液蛋白定性、尿液蛋白定量以及尿液中轻链含量等检测。结果 54例轻链型单克隆免疫球蛋白血症中,属κ轻链型的有24例,占44%,尿液中κ轻链含量为(1.86±1.77)g/L,κ/λ值为(277.22±253.56);属λ轻链型的有30例,占56%,尿液中λ轻链含量为(0.70±0.58)g/L,κ/λ值为(0.03±0.02);54例患者血清蛋白电泳中仅有12例在α2～γ区间有淡染的M带出现;患者的尿液均呈本-周蛋白阳性,其中30例尿蛋白定性呈阴性或弱阳性,其余24例尿蛋白定性呈+～2+,尿蛋白定量为0.2～8.6g/L之间。结论轻链型单克隆免疫球蛋白血症患者血清蛋白电泳结果的M蛋白检出率低,尿液中蛋白多为低浓度,因此利用高灵敏度的免疫固定技术检测患者的血清及尿液中的游离轻链,能够提高该病患者M蛋白的检出率。Objective To study and analyze body fluid protein characteristics of 54 patients with light chain type of monoclonal gammopathy.Methods Serum protein electrophoresis（SPE）,qualitative and quantitative tests of urine protein were performed,urine bence jones protein（BJP） and light chain type of protein and so on.were determined in 54 cases from 75 cases of light chain type,which were screened out from 2 765 cases of monoclonal gammopathy patients with immunofixation electrophoresis（IFE）.Results In 54 patients with light chain type of monoclonal gammopathy,there were 24 cases of κ light chain type,accounting for 44%,in which protein of κ light chain type was（1.86±1.77） g/L,κ/λ value was（277.22±253.56） in urine;24 cases of λ light chain type,accounting for 56%,in which protein of λ light chain type was（0.70±0.58） g/L,κ/λ value was（0.03±0.02） in urine.In 54 patients with light chain type of monoclonal gammopathy,light M-band appeared in α2-γ regions in 12 cases with Serum protein electrophoresis.All urine samples were found BJP positive,of which 30 were found negative or weakly positive,and 24 positive ＋-2＋ with urine qualitative test.Results of urine quantitative test were 0.2-8.6 g/L.Conclusion The SPE detection rate of M protein is low in patients with light chain type of monoclonal gammopathy,which the urine protein content is mostly low concentration.Therefore,the M protein detection rate can be improved by detect free light chain in patients′ serum and urine with highly sensitive IFE.

关 键 词：单克隆免疫球蛋白血症 血蛋白质类 免疫学技术 

分 类 号：R55[医药卫生—血液循环系统疾病]