梅州市首次C群流行性脑脊髓膜炎聚集性病例病原学分析  

Etiological analysis of the first gathered cases of epidemic cerebrospinal meningitis caused by serogroup C neisseria meningitis in Meizhou city

作　　者：陈志青[1] 刘美真[2] 蔡芳华[1] 曾丽珍[1] 古汉福[3] 肖胜传[1] 

机构地区：[1]梅州市疾病预防控制中心,广东梅州514071 [2]广东省疾病预防控制中心,广东广州510300 [3]梅州市人民医院,广东梅州514031

出　　处：《国际检验医学杂志》2013年第13期1662-1663,共2页International Journal of Laboratory Medicine

摘　　要：目的对梅州市首次C群流行性脑脊髓膜炎(流脑)聚集性病例进行病原学分析。方法对梅州市首次C群流脑聚集性病例进行流行病学调查,标本采集,菌株分离、鉴定、血清学分群及药物敏感性检测,并进行多位点序列分型(MLST)分析。结果梅州市首次从2例流脑病例中分离到2株C群脑膜炎奈瑟菌(Nm),第2例病例脑脊液标本PCR检测出C群siaD基因阳性。第2例及17例密切接触者咽拭子标本均未检出脑膜炎奈瑟菌。菌株对环丙沙星、萘啶酸、甲氧苄啶/磺胺甲恶唑耐药,但对头孢噻肟、头孢曲松、美罗培南、阿奇霉素、咪诺霉素、氯霉素、利福平、青霉素、氨苄西林敏感。首例患者分离菌株MLST分型为ST-4821型。结论此为近年梅州市首次报告的C群流脑聚集性疫情,提示应加强对C群奈瑟菌的监测,密切关注C群流脑的发病及流行趋势。Objective To analyze the etiology of first gathered cases of epidemic cerebrospinal meningitis（ECM）caused by serogroup C neisseria meningitis（Nm）in Meizhou city.Methods The first gathered cases of serogroup C ECM in Meizhou city were analyzed by epidemiological investigation,specimens collecting,strains isolation,identification,serological grouping,antimicribiol susceptibility test and Multilocus sequence typing（MLST）.Results Two serogroup C Nm strains were isolated from two cases in Meizhou city first.Nothing isolated from the second case and 17 close contacts.PCR identified siaD（C）gene was positive from the second case cerebrospinal fluid.The strain resistant to ciprofloxacin,nalidixic acid,trimethoprim/sulfamethoxazole,but sensitive to cefotaxime,ceftriaxone,meropenem,azithromycin,cefminox,chloramphenicol,rifampin,penicillin and ampicillin.MLST of the first strain was type ST-4821.Conclusion This is the first gathered cases of ECM caused by serogroup C Nm in Meizhou city.Serogroup C Nm monitoring,serogroup C ECM incidence and prevalence trend should be strengthen.

关 键 词：脑膜炎 脑膜炎球菌性 聚合酶链反应 奈瑟球菌 脑膜炎 血清C群 

分 类 号：R515[医药卫生—内科学]