致婴幼儿腹泻的肠致病性大肠埃希菌血清分型和毒力基因检测  

Serotyping of the infant diarrhea causing Enteropathogenic Escherichia coli and their virulence genes detection

作　　者：潘伟光[1] 李一凡[1] 邓启文[1] 张健华[1] 

机构地区：[1]广东医学院附属南山医院微生物检验室,广东深圳518052

出　　处：《国际检验医学杂志》2013年第13期1691-1692,1695,共3页International Journal of Laboratory Medicine

摘　　要：目的了解引起婴幼儿腹泻的肠致病性大肠埃希菌血清分型情况及其毒力基因携带状况。方法采集2011年6月至2012年2月医院儿科婴幼儿腹泻患者的粪便标本进行大肠埃希菌的分离培养,通过血清凝集试验和细菌生化反应筛选出肠致病性大肠埃希菌,采用多重PCR检测毒力基因escV、bfpA、Stx1和Stx2。结果 503例粪便标本中共分离出47株肠致病性大肠埃希菌,检出率是9.34%。血清分型有9种,最多的血清型是O86:k61,占了阳性菌株的27.66%。毒力基因escV阳性合计22株,bfpB阳性合计17株,检出率分别是46.81%和36.17%,其中,(escV+bfpB)阳性14株,escV阳性8株,bfpB阳性3株,携带毒力基因的菌株阳性率为53.19%,未检出毒力基因Stx1和Stx2。结论肠致病性大肠埃希菌血清学分型与毒力基因携带有差异,有条件的实验室可以直接检测大肠埃希菌的毒力基因,为临床提供更准确的诊断依据。Objective To investigate of the serogroup distribution of Enteropathogenic Escherichia coli that cause diarrhea in infants and young children and the presence of their virulence genes.Methods Isolated and cultured Escherichia coli from the stool specimens of pediatric diarrhea patients,collected in our hospital from June 2011 to February 2012.Then screened the Enteropathogenic Escherichia coli using serum agglutination test and bacterial biochemical reactions.Multiplex PCR was performed to detect virulence genes escV,bfpA,Stx1 and Stx2.Results 47 Enteropathogenic Escherichia coli was isolated from 503 diarrhea stool samples,with a detection rate of 9.34%.There were 9 serotypes,in which O86：k61 wa most common,with detection rates of 27.66%.The number of samples for escV positive and bfpB po-sitive were 22（detection rate 46.81%） and 17（detection rate 36.17%）,respectively.Within these,sample number for escV＋bfpB double positive was 14,escV positive was 8 and bfpB positive was 3.The positive rate of strains carrying virulence genes of 53.19%.Virulence genes Stx1 and Stx2 were not detected.Conclusion There is a difference between the serotyping of Enteropathogenic Escherichia coli.and their virulence genes carrying status.It is advisable that laboratory with better conditions should directly detect Enteropathogenic Escherichia coli virulence genes to provide the more precise information for clinical diagnosis.

关 键 词：肠致病性大肠杆菌 腹泻 血清 毒力 

分 类 号：R440[医药卫生—诊断学]