咯利普兰治疗大鼠脊髓横断损伤的实验研究  

Effects of Rolipram on Spinal Cord Transection Injury in Rats

作　　者：李锋涛[1] 程斌[2] 李浩鹏[1] 臧全金[1] 张廷[1] 贺西京[1] 

机构地区：[1]西安交通大学第二附属医院骨二科,陕西西安市710004 [2]西安交通大学第二附属医院骨三科,陕西西安市710004

出　　处：《中国康复理论与实践》2013年第7期628-630,共3页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的探索咯利普兰治疗大鼠脊髓横断损伤的可行性。方法取健康成年雌性Sprague-Dawley大鼠30只,随机分为假手术组(Sham组)、损伤组(SCI组)、咯利普兰治疗组(R组),每组10只。R组建立大鼠脊髓横断损伤模型后立刻皮下注射咯利普兰;Sham组仅打开椎板;SCI组及Sham组皮下注射相同体积二甲基亚砜(DMSO)。于损伤后2、4、6、8周采用BBB评分法观察大鼠后肢运动功能情况,损伤后2周时应用免疫组织化学染色检测各组生长相关蛋白-43(GAP-43)及胶质细胞酸性蛋白(GFAP)的表达。结果损伤后6周、8周时,R组BBB评分优于SCI组(P<0.05)。损伤后2周,R组GAP-43表达高于SCI组(P<0.05),GFAP表达量低于SCI组(P<0.05)。结论咯利普兰能提高大鼠脊髓横断损伤神经功能评分,提高GAP-43表达,抑制GFAP表达。Objective To investigate the possibility of rolipram for treatment of spinal cord injury（SCI） in rats.Methods 30 adult female Sprague-Dawley rats were divided into sham-operation group（sham group,n=10）,spinal cord injury group（SCI group,n=10） and rolipram treatment group（R group,n=10）.The rats in SCI group and R group were modeled as spinal cord transection injury,and R group was administrated with rolipram subcutaneouly after SCI.They were assessed with Basso Beattie and Bresnahan（BBB） score 2,4,6,and 8 weeks after SCI,and the expressions of growth associated protein 43（GAP-43） and glial fibrillary acidic protein（GFAP） were detected with immunohistochemistry 2 weeks after SCI.Results There were significant difference in the BBB scores between SCI and R groups 6 and 8 weeks after SCI（P＆lt;0.05）.The expression of GAP-43 was more and GFAP was less in R group than in SCI group（P＆lt;0.05）.Conclusion Rolipram can increase the expression of GAP-43 and inhibit the expression of GFAP,while improves the the motor function in rats after spinal cord transsection injury.

关 键 词：脊髓损伤 咯利普兰 运动功能 生长相关蛋白43 胶质细胞酸性蛋白 大鼠 

分 类 号：R681.2[医药卫生—骨科学]