实时监测溶酶体光损伤诱导细胞凋亡过程中Bax定位的时空变化  

Real-Time Monitoring of Bax Subcellular Localization during Apoptosis Induced by Lysosomal Photodamage

作　　者：刘镭[1] 张真真[1] 

机构地区：[1]华南师范大学生物光子学研究院激光生命科学研究所,暨激光生命科学教育部重点实验室,广东广州510631

出　　处：《激光生物学报》2013年第3期210-213,共4页Acta Laser Biology Sinica

基　　金：科技部国家重点基础研究973计划项目(2011CB910402;2010CB732602);国家自然科学基金项目(31101028)

摘　　要：光敏剂N-aspartyl chlorin e6(NPe6)能特异性定位于溶酶体,溶酶体光损伤光能触发线粒体凋亡通路从而诱导细胞凋亡。Bax是Bcl-2家族一员,是调控细胞凋亡的关键因子之一。静息态下,Bax定位于细胞质中;而在细胞凋亡过程中,Bax会从细胞质转位到线粒体,损伤线粒体,从而启动细胞凋亡。在本研究中,我们在活细胞内实时监控溶酶体光损伤诱导细胞凋亡过程中Bax亚细胞定位的动态变化。结果表明,溶酶体光损伤后约170 min,Bax开始转位到线粒体,在30 min之内便大量聚集在线粒体上。该研究结果实时动态地展示了细胞凋亡过程中Bax的时空变化过程。Photodynamic therapy （PDT） employing photosensiter N-aspartyl chlorin e6 （NPe6） can induce lysosome disruption and initiate the intrinsic apoptotic pathway. Bax, a member of the Bcl-2 family of proteins, is an essential reg- ulator of apoptosis. Bax is normally found in the cytosol of healthy cells, while it translocates to mitochondria in response to many apoptotic stimuli. In this study, using real-time single-cell analysis, we have investigated the kinetics of Bax distribution during NPe6-induced apoptosis in ASTC-a-1 cells. The results show that Bax redistribution occurred at about 170 min after treated with NPe6-PDT, and then sequestered into clusters associated with the mitochondira within 30 min. Our data clearly showed the spatial and temporal changes in Bax distribution in living cells during NPe6-induced apoptosis.

关 键 词：溶酶体光损伤 NPe6 BAX 细胞凋亡 

分 类 号：Q255[生物学—细胞生物学]