柞蚕14-3-3基因的鉴定及表达分析(英文)  

Characterization and Expression of 14-3-3 Gene from the Wild Silkworm,Antheraea pernyi.

作　　者：张晓[1] 付维维[1] 王磊[1] 朱保建[1] 刘秋宁[1] 戴立上[1] 孙钰[1] 刘朝良[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036

出　　处：《激光生物学报》2013年第3期237-242,共6页Acta Laser Biology Sinica

基　　金：supported by the earmarked fund for Modern Agro-industry Technology Research System(CARS-22-SYZ10);National 863 plans projects of China(2011AA100306);Natural Science Foundation of Anhui Province of China(11040606M98)

摘　　要：14-3-3蛋白是一种可以改变其结合蛋白构象的酸性蛋白质。柞蚕14-3-3 cDNA序列全长1 220 bp,包括一个126 bp的5'非编码区和一个350 bp的3'非编码区。该基因的开放读码框长度为744 bp,编码247个氨基酸。序列比对结果表明,柞蚕14-3-3蛋白与家蚕的14-3-3蛋白具有高度同源性。此外对柞蚕14-3-3基因进行了原核表达和重组蛋白纯化。SDS-PAGE和免疫印迹结果表明,分子量大小约32 kD的重组蛋白在大肠杆菌中得到了成功表达。The 14-3-3 protein is a acidic regulatory protein modulating the conformation of its binding partner. A 14-3- 3 gene, named as Ap-14-3-3, was identified from the Chinese Oak Silkworm Antheraea pernyi. The full-length cDNA of Ap-14-3-3 is 1 220 bp, including a 5＇-untranslated region （UTR） of 126 bp, 3＇-UTR of 350 bp and an open reading frame （ORF） of 744 bp encoding a polypeptide of 247 amino acids. The deduced A. pernyi 14-3-3 protein sequence is highly homologous to its homologue of Bombyx mort. Prokaryotic expression and purification of the Ap-14-3-3 protein were performed, SDS-PAGE and western blot analysis demonstrated that a 32 kD recombinant protein was successfully expressed in E. coli cells.

关 键 词：柞蚕 14-3-3基因 表达 

分 类 号：Q786[生物学—分子生物学]