紫外吸收法、电导率法检验大白菜种子活力的研究  

Analysis on Seed Vigor Testing of Chinese Cabbage Base on UV Absorbance Method and Electrical Conductivity Method

作　　者：刘春香[1] 隋铭[1] 胡畔[1] 赵卫星[1] 

机构地区：[1]山东省高校生物化学与分子生物学重点实验室(潍坊学院),山东潍坊261061

出　　处：《种子》2013年第7期18-21,共4页Seed

基　　金：山东省自然基金项目资助(编号:2009ZR07021);潍坊学院青年基金项目(编号:2013Z10)

摘　　要：通过人工老化种子和不同年代种子具有的活力不同,以发芽指数为活力参考标准来探索种子浸泡液电导率测定法和紫外吸收测定法是否适合大白菜种子活力的快速检测。结果表明:大白菜种子紫外区随着波长的缩短吸收值增大,但经玻璃比色杯滤过后会在286 nm下显示吸收峰,随着种子活力的降低286 nm下的OD值呈上升趋势,对发芽率高于85%的种子适用,而对于活力过低的种子检验不可靠;随着种子活力的降低电导率呈上升趋势,但对活力差异较小的种子间检验不可靠。因此,对发芽率合格范围内的种子可以根据286 nm(玻璃比色皿)紫外吸收值评价大白菜种子活力的高低,其他的种子可参考电导率值比较种子活力。UV absorbance method and electrical conductivity method of seed soaking solution were investigated to find which one is fit for quick seed vigor test using artificial aged chinese cabbage seeds and natural aged seeds with different seed vigor.The results shows that seed soaking solution absorption value is increasing with the shorten of wavelength in quartz cup,has a absorption peak on 286 nm wavelength in glass cup.The OD values of 286 nm absorption is increasing with the decline of seed vigor in the region of germinating rate over 85%,this trend is unfit for seeds germinating rate below 85%;however elcetrical conductivity method is fit for low seed vigor testing,but when seeds have relatively little difference on vigor level,this method is unreliable.So as for as qualified germinating rate seeds is concerned,286 nm absorbance peak testing method is fit for seed vigor testing,while electrical conductivity testing is fit for others.

关 键 词：紫外吸收 电导率 大白菜 种子活力 

分 类 号：S634.1[农业科学—蔬菜学]