α-1,6-甘露糖转移酶(Och1p)缺陷型突变株毕赤酵母X33生长条件优化  

Growth condition optimization of α-1,6-mannose transferase(Och1p) deficient mutant strain of pichia pastoris X33

作　　者：李萌[1] 朱一菲 张大成 朱瑞宇 金坚[1,2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,无锡214122 [2]江南大学医药学院,无锡214122

出　　处：《工业微生物》2013年第4期71-76,共6页Industrial Microbiology

基　　金：江苏省基础研究计划(自然科学基金)资助项目(BK2009071);国家自然科学基金(81101667)

摘　　要：为促进Pichia pastoris X33α-1,6甘露糖转移酶(Och1p)突变株(och1 mX33)作为蛋白表达宿主菌的应用,提高Pichiapastoris X33突变株的生长速度,本研究对影响酵母生长条件中的接种量、通气量、培养基pH值、碳源含量(甘油含量)、外界渗透压(培养基中加入山梨醇)及培养温度各因素进行研究,发现pH 5.0、培养基中添加0.5 mol/L山梨醇的单因素条件可提高Pichia pastoris X33突变菌株的生长速度,提高Pichia pastoris X33突变株作为宿主菌应用的可行性。In order to improve the growth rate of Pichia pastoris X33 mutant strain, pH of medium, carbon source content （ and temperature that would affect the glycerol content）, osmotic pressure outside the parameters of inoculum, aeration, （medium supplemented with sorbitol） of Pichia pastoris were investigated. It was found that under the single factor condition the growth rate of Pichia pastoris X33 mutant strain would substantially increase if the culture medium was added with 0.5 mol/L sorbitol at pH 5.0, which would promote the application of Pichia pastoris X33 mutant strain as a host to express the heterologous protein.

关 键 词：糖基化 生长条件 优化 α-1 6-甘露糖转移酶 

分 类 号：Q93-3[生物学—微生物学]