大孔树脂纯化红花中羟基红花黄色素A的产业化探索  

Preliminary Exploration of Industrialization for Purification of Hydroxysafflor Yellow A from Carthamus tinctorius by Macroporous Resin

作　　者：吴建雄[1,2,3] 秦建平[1,2,3] 万琴[1,2,3] 王振中[1,2,3] 萧伟[1,2,3] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001 [3]江苏省企业院士工作站,江苏连云港222001

出　　处：《中国实验方剂学杂志》2013年第16期5-7,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部"重大新药创制"项目(2011ZX09401-097)

摘　　要：目的:优选D101大孔树脂纯化红花中羟基红花黄色素A的工业化放大工艺。方法:采用HPLC测定羟基红花黄色素A含量,通过单因素试验考察上样液pH、洗脱剂体积分数和用量、药材-树脂质量比对纯化工艺的影响,通过中试及工业化生产验证优选的纯化工艺。结果:优选的纯化工艺条件为上样液pH 4.0,药材-树脂质量比1∶6,加4 BV水以3 BV.h-1的流速洗脱除杂,用5%乙醇6 BV以3 BV.h-1的流速洗脱,收集洗脱液。结论:该工艺简单可行、分离效果好,适用于羟基红花黄色素A的大生产。Objective： To optimize industrialization amplification process for purification of hydroxysafflor yellow A from Carthamus tinctorius by macroporous resin. Method： The content of hydroxysafflor yellow A was determined by HPLC, single factor tests were adopted to investigated effects of pH of sample solution, the concentration and amount of eluant, quality ratio of raw materials to resin on purification technology, then verified optimized technology through pilot and industrialization production. Result： Optimum purification conditions were as follows ： ratio of crude drug to D101 resin 1 ： 6, pH of sample solution 4.0, eluted impurity with 4 BV water at flow rate of 3 BV-h-1, then eluted with 6 BV 5% ethanol at 3 3 BV -h-1, collected eluent. Conclusion： This technology was simple and feasible with good separation, it could be suitable for industrialized production of hydroxysafflor yellow A.

关 键 词：羟基红花黄色素A 大孔树脂 纯化 产业化 

分 类 号：R284.2[医药卫生—中药学] R283.6[医药卫生—中医学]