草乌净制机制探析  

Analysis of Cleansing Mechanism of Aconitum kusnezoffii

作　　者：侯跃飞[1] 杜红[1] 王哲[1] 刘红玉[1] 李飞[1] 谭鹏[1] 杨蕾[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2013年第16期45-47,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中医药行业科研专项(201107008);北京中医药大学自主选题(2011-MZYY-07);国家自然科学基金项目(81102807)

摘　　要：目的:探析草乌的净制机制。方法:采用HPLC测定3批带残茎草乌、去残茎草乌及草乌残茎中新乌头碱、乌头碱、次乌头碱、苯甲酰新乌头碱、苯甲酰乌头碱、苯甲酰次乌头碱的含量。结果:带残茎草乌、去残茎草乌、草乌残茎中3种双酯型生物碱的总含量分别是0.59%～0.66%,0.61%～0.69%,0.39%～0.42%;3种单酯型生物碱的总含量分别是0.09%～0.10%,0.09%～0.12%,0.06%～0.08%。草乌不同部位的色谱图中除6种酯型生物碱峰外还存在共有峰4,残茎中峰4的峰面积是去残茎草乌的55%～80%。结论:草乌残茎和去残茎草乌中6种生物碱成分含量存在差异;残茎属于生物碱成分含量较低的非药用部位,需去除。Objective： To analyze cleansing mechanism of Aconitum kusnezoffii. Method： HPLC was adopted to determine the content of mesaconine, aconitine, hypaconitine, benzoylmesaconine, benzoylaconitine, benzoylhypaconitine in A. kusnezoffii with stems, A. kusnezoffii without stems and stems of A. kusnezoffii. Result： The total content of three kind of diester alkaloids in A. kusnezoffii with stems, A. kusnezoffii without stems and stems ofA. kusnezoffii was 0. 59% -0. 66% , 0. 61% -0.69% , 0. 39% -0.42% , respectively; The total content of three kind of monoester alkaloids was 0. 09%-0. 10%, 0. 09% -0. 12% , 0. 06%-0. 08% , respectively. Except six alkaloids chromatographic peaks, there was another common peak （peak 4） , area of peak 4 in stems of A. kusnezoffii was 55%-80% to that in A. kusnezoffii without stems. Conclusion： Content of six alkaloids was foundin A. kusnezoffii without stems and stems of A. kusnezoffii was different. Stems of A. kusnezoffii was the non- medicinal parts whose content of six alkaloids was low, it could be removed.

关 键 词：草乌 净制 残茎 高效液相色谱 生物碱 

分 类 号：R283.3[医药卫生—中药学]