道地产区不同品种怀地黄中梓醇及毛蕊花糖苷的含量比较  

Content Comparison of Catalpol and Acteoside in Rehmannia glutinosa with Different Varieties from Genuine Producing Area

作　　者：杨云[1] 张华锋[1] 刘炯[1] 张杰[1] 刘富岗[1] 卫冰[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2013年第16期97-101,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：地黄规范化种植基地优化升级及系列产品综合开发研究(2011BAIO06B02)

摘　　要：目的:考察道地产区不同品种怀地黄中梓醇及毛蕊花糖苷的含量。方法:采用高效液相色谱法,甲醇回流提取梓醇及毛蕊花糖苷,Dikma Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流速1.0 mL.min-1,柱温30℃,梓醇流动相为乙腈-0.1%磷酸(1∶99),检测波长210 nm,毛蕊花糖苷流动相为乙腈-0.1%醋酸(16∶84),检测波长334 nm。结果:道地产区不同品种怀地黄中梓醇及毛蕊花糖苷含量不同,鲜地黄与生地黄中梓醇及毛蕊花糖苷的含量也不相同。结论:怀地黄品种品质不一,有必要进行种质资源优选。Objective： To investigate the content of catalpol and acteoside in Rehmannia glutinosa with different varieties from genuine producing area. Method： Catalpol and acteoside were reflux extraction with methanol and determined by HPLC. The chromatographic column was Dikma Diamonsil C18 （4.6 mm × 250 mm, 5 μm） ; the flow rate was 1.0 mL .min-2 ; the column temperature was kept at 30 ℃ ; the mobile phase consisted of aeetonitrile-0. 1% phosphoric acid solution （1：99） and the detection wavelength was set at 210 nm for catalpol; the mobiles phase consisted of acetonitrile-0. 1% acetic acid solution （16：84） and the detection wavelength was set at 334 nm for acteoside. Result： The content of catalpol and acteoside was different in different varieties from genuine producing area and the differences also exist in the fresh and dried R. glutinosa. Conclusion： The quality of varieties of R. glutinosa was different and it was necessary to screen the optimal variety.

关 键 词：道地产区 品种 梓醇 毛蕊花糖苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]