款冬花传统规格等级与芦丁含量的相关性分析  

Relativity Analysis of Rutin Content and Traditional Specification Drade of Farfarae Flos

作　　者：凌珊[1] 易炳学[1] 龚千锋[1] 肖永庆[2] 

机构地区：[1]江西中医学院,南昌330004 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2013年第16期147-150,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药行业科研专项课题(201007012-2-9)

摘　　要：目的:探讨款冬花传统规格等级方法与芦丁含量的相关性。方法:采用HPLC测定不同等级款冬花中芦丁含量,Dikma Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-1%乙酸(20:80),检测波长254 nm。建立不同等级款冬花的HPLC指纹图谱并进行相似度分析,流动相乙腈-0.1%磷酸水溶液梯度洗脱,检测波长240 nm。结果:优级款冬花样品中芦丁平均含量略高于统货样品,但二者差别不大;不同等级、产地与批次的生品与炮制品款冬花的指纹图谱相似度均>0.85,优级样品共有12个相对峰面积平均值大于统货样品。结论:建立的款冬花中芦丁含量测定及指纹图谱方法简单、重复性好。传统分级方法具有一定合理性,但芦丁含量高低与传统等级相关性不大,不应将其作为款冬花饮片分级的主要指标。Objective： To investigate relativity between the content of rutin and traditional specification grade of Farfarae Flos. Method： The content of turin from Farfarae Flos was performed by HPLC on Dikma Diamonsil C18column （4.6 mm ×250mm,5μm） with acetonitrile -1% acetic acid （20：80） as mobile phase and detection wavelength at 254 nm. HPLC fingerprint and similarity analysis of different specifications of Farfarae Flos was performed on Dikma Diamonsil C18column （4.6 mm ×250mm,5μm） with acetonitrile -0. 1% H3PO4gradient elution and detection wavelength of 240 nm. Result ： Average content of rutin in superior grade was slightly higher than gradeless samples with little difference; Similarity of fingerprint of Farfarae Flos from different grades, habitats and batches was more than 0. 85. Average values of twelve relative peak area of superior samples were larger than that of gradeless samples. Conclusion： Quantification method of rutin and fingerprint analysis was feasible and reproductive. Traditional classification of Farfarae Flos had a certain rationality, but the content of rutin had little correlation with traditional classification, so to classificate Farfarae Flos with the content of rutin as main index was unreasonable.

关 键 词：款冬花 传统分级 芦丁 高效液相色谱法 指纹图谱 

分 类 号：R282.7[医药卫生—中药学] R282.6[医药卫生—中医学]