HPLC同时测定广藿香中雷杜辛黄酮醇及广藿香酮的含量  

Simultaneous Determination of Retusine and Pogostone in Pogostemon cablin by HPLC

作　　者：张颖梅 陈海明[2] 吴晓丽[2] 苏祖清 曾惠芳[3] 苏子仁[2] 

机构地区：[1]广州王老吉药业股份有限公司,广州510450 [2]广州中医药大学新药开发研究中心,广州510006 [3]广州中医药大学第一附属医院,广州510405

出　　处：《中国实验方剂学杂志》2013年第16期160-163,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立高效液相色谱(HPLC)同时测定广藿香中雷杜辛黄酮醇和广藿香酮的方法,对11批市售的广藿香药材进行质量分析。方法:采用高效液相色谱法。Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.2%甲酸溶液(65∶35),等度洗脱;检测波长310 nm,流速0.8 mL.min-1,柱温30℃。结果:雷杜辛黄酮醇在0.020～0.36μg,广藿香酮在0.025～0.40μg呈良好线性关系,相关系数均为1.000 0,平均回收率雷杜辛黄酮醇为98.57%(RSD 2.06%),广藿香酮为100.04%(RSD 2.66%);11批样品中雷杜辛黄酮醇含量在1.13～8.72 mg.g-1,广藿香酮含量在1.19～4.38 mg.g-1。结论:方法简便、快速、准确,重复性好,可用于广藿香中雷杜辛黄酮醇及广藿香酮含量的同时测定。Objective： To establish a high performance liquid chromatography （HPLC） method for simultaneous determination of retusine and pogostone in Pogostemon cablin, and analyze the quality of 11 batches of retusine and pogostone in P. cablin. Method： The RP-HPLC system consisted of a Phenomenex Luna C18 column （4.6 mm × 250 mm, 5 μm） and the mobile phase of acetonitrile-formic acid by isocratic elution. The flow rate was set at 0. 8 mL -min-1 and detected at 310 nm; the column temperature was kept at 30 ℃. Result： The linear regression for retusine and pogostone was obtained in the range of 0. 020-0.36 μg （r = 1. 000 0 ） and 0. 025-0.40 μg （r = 1. 000 0） respectively; the average recovery was 98.57% （RSD 2.06% ） and 100. 04% （RSD 2.66% ） respectively. The content of retusine and pogostone in 11 batches of P. cablin were in the range of 1.13-8.72 mg · g-1 and 1.19-4.38 mg ·g-1 respectively. Conclusion： The method is quick, simple and reproducible for the determination of retusine and pogostone in P. cablin simultaneously.

关 键 词：广藿香 高效液相色谱法 雷杜辛黄酮醇 广藿香酮 

分 类 号：R284.1[医药卫生—中药学]