中药臭灵丹中洋艾素和金腰乙素的含量测定  

Determination of Artemitin and Chrysosptertin B in Laggera pterodonta

作　　者：李书华[1] 张智锦[1] 刘芳[1] 

机构地区：[1]乐山师范学院化学与生命科学学院,四川乐山614004

出　　处：《中国实验方剂学杂志》2013年第16期168-170,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省旅游发展研究中心项目(LYM12-36);四川省教育厅科研项目(13ZA0105)

摘　　要：目的:建立同时测定臭灵丹中洋艾素和金腰乙素含量的高效液相色谱方法。方法:采用高效液相色谱法,Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水(60∶40),流速1.0 mL.min-1,检测波长257 nm,柱温32.2℃。结果:洋艾素和金腰乙素分别在0.448～2.24和0.352～1.76μg线性关系良好,平均回收率洋艾素为103.48%(RSD 0.87%),金腰乙素为103.42(RSD 0.91%)。结论:该方法简便,重复性好,可以用于同时测定臭灵丹中洋艾素和金腰乙素的含量。Objective： To establish an effective HPLC method for determining the content of artemitin and chrysosptertin B in Laggera pterodonta. Method： By HPLC, a Diamonsil C18 column （4.6 mm × 250 mm, 5 μm） was adopted with methanol-water （60： 40） as the mobile phase for elution at the flow rate of 1.0 mL ·min-1 The detection wavelength was set at 257 nm and the temperature was kept at 32.2℃. Result： Good linearity was obtained in the range of 0. 448-2.24 and 0. 352-1.76 μg for artemitin and chrysosptertin B respectively. The method recoveries of artemitin and chrysosptertin B were 103.48% and 103.42% , RSDs were 0. 87% and 0.91% respectively. Conclusion： The method is simple, accurate and suitable for the determination of artemitin and chrysosptertin B, which can be used for quality control of Laggera pterodonta.

关 键 词：臭灵丹 洋艾素 金腰乙素 含量 

分 类 号：R284.1[医药卫生—中药学]