UPLC测定肝微粒体中柚皮苷和柚皮素含量  

Determination of Naringin and Naringenin in Rat Liver Microsomes by UPLC

作　　者：林靖然[1] 邓少东[1] 肖凤霞[1] 林励[1] 邓韬[1] 张旭倩[1] 

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《中国实验方剂学杂志》2013年第16期175-178,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部"十二五"科技支撑计划项目(2011BAI01B02);广东省科技计划项目(2012A030100005);茂名市科技重大专项项目(2012A01002)

摘　　要：目的:建立UPLC测定大鼠肝微粒体中柚皮苷和柚皮素含量的方法,并初步考察二者于不同时间点的代谢情况。方法:采用UPLC色谱系统,ACQUITY BEH C18色谱柱(3.0 mm×100 mm,1.7μm),流动相甲醇-0.1%乙酸水溶液梯度洗脱,流速0.5 mL.min-1,柱温30℃,检测波长283,289 nm,内标为槲皮素,进样量3μL。结果:柚皮苷及柚皮素均与槲皮素分离良好且无内源性干扰。生物样品中柚皮苷和柚皮素的线性范围分别为3.814～38.143(r=0.999 2),3.586～35.857 mg.L-1(r=0.999 6);柚皮苷的方法回收率95.43%～97.95%,绝对回收率97.33%～98.18%;柚皮素的方法回收率95.27%～99.31%,绝对回收率97.71%～99.73%;柚皮素在体外肝微粒体中的I相代谢较柚皮苷显著。结论:该方法快速、灵敏、准确,可用于大鼠肝微粒中柚皮苷和柚皮素的含量测定。Objective ： To establish a method for determining the content of naringin and naringenin in rat liver microsomes by UPLC, and preliminary investigation in vitro metabolism of two ingredients at different times points. Method： Determination was performed on UPLC chromatographic system, ACQUITY BEH C18 column （3.0 mm × 100 mm, 1.7 μm） with a mobile phase of methanol-0. 1% acetic acid and gradient elution at a flow rate of 0.5 mL -min-1, detection wavelength was 283, 289 nm, column temperature was 30 ℃ , with quercetin as an internal standard, injection volume was 3 μm. Result： Naringin, naringenin and internal standard quercetin had good separation degree and no endogenous interference. The linear range of naringin and naringenin were 3. 814-38. 143 （r =0. 999 2） , 3. 586-35. 857 mg ·L-1 （r =0. 999 6） , respectively. Method recoveries of naringin ranged from 95.43% to 97.95% and absolute recoveries ranged from 97.33% to 98.18%. Method recoveries of naringenin ranged from 95.27% to 99.31% and absolute recoveries ranged from 97.71% to 99.73%. I phase metabolism of naringenin in in vitro liver microsomes was more significant than naringin. Conclusion： This method was rapid, sensitive and accurate, it could be used for determination of naringin and naringenin in rat liver microsomes.

关 键 词：柚皮苷 柚皮素 大鼠 肝微粒体 超高效液相色谱法 

分 类 号：R945[医药卫生—微生物与生化药学]