甘草次酸对大鼠山奈酚血浆浓度的影响  

Effect of Glycyrrhetinic Acid on the Plasma Concentration of Kaempferol in Rats

作　　者：覃苑[1] 周能[1,2] 龚琦[1] 周振[2] 

机构地区：[1]广西大学化学化工学院,南宁530004 [2]玉林师范学院化学与材料学院,广西玉林537000

出　　处：《中国实验方剂学杂志》2013年第16期183-187,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81060360);广西科学基金(2010GXNSFA013065);2010年广西高等学校优秀人才资助计划项目

摘　　要：目的:探索甘草次酸对大鼠山奈酚血浆浓度的影响。方法:将78只SD大鼠,体重150～210 g,随机分成4组,雌雄各半,即实验高浓度组与低浓度组,及高浓度与低浓度对照组。每组中又包括3个小组(即1,2,3 h)。高浓度实验组灌胃50 mg.kg-1山柰酚+50 mg.kg-1甘草次酸,低浓度实验组灌胃25 mg.kg-1山奈酚+50 mg.kg-1甘草次酸。对照组只灌胃山奈酚,剂量与实验组相同。灌胃给药后分别于1,2,3 h用摘眼球取血法取血。血浆在90℃水浴中,加盐酸水解120 min,然后以乙腈为萃取剂,超声波为辅助提取,利用高效液相色谱(HPLC)测定血浆中山奈酚的浓度。结果:大鼠口服山奈酚后在血浆未能检测到游离山奈酚。低浓度组合药和单药灌胃对山奈酚血浆浓度没有影响;高浓度的合药组中山奈酚浓度(水解后)明显高于单药组(P<0.05)。结论:在高浓度组中,甘草次酸能够提高山奈酚的血浆浓度。Objective： To study the effect of glycyrrhetinic acid on the plasma concentration of kaempferol in rats. Method： Seventy-eight SD rats, weighing 150-210 g, half male and half female, were distributed into 4 groups, including control group of high concentration and low concentration, experimental group of high concentration and low concentration. Then each group was divided into three sub-groups, namely group of distribution phase （ 1 h ）, equilibrium phase （ 2 h ） and elimination phase （ 3 h ）. Experimental group of high concentration and low concentration was administered by garage at a dose of 50 mg .kg-i kaempferol mixed with 50 mg ·kg-1 grycyrrhetinic acid and 25 mg ·kg-1 mixed with 50 mg ·kg-1 grycyrrhetinic acid, respectively. The control group as administered by gavage at a same dose like the experimental group except grycyrrhetinic acid. Plasma samples were collected by picking eyeballs 1, 2, 3 h after administration. Plasma was hydrolyzed at 90℃ for 120 min with hydrochloric acid. Then they were extracted with acetonitrile and concentration of kaempferol was determined by HPLC. Result： No free kaempferol was detected in rat＇ s plasma after administration of kaempferol. The results showed that at two concentration levels there was no significant difference between two sets data of experimental group and control group, but there was significant difference （ P 〈 0.05 ） between two group athigh concentration level in plasma （P 〈 0.05）. Conclusion： Grycyrrhetinic acid can affect the pharmacokinetics of kaempferol in plasma at high concentration level.

关 键 词：甘草次酸 山奈酚 血浆 HPLC 

分 类 号：R285.5[医药卫生—中药学]