复方蛹虫草颗粒对小鼠免疫功能以及脾淋巴细胞增殖的影响  

Effects of Compound Cordyceps Militaris on Immunity Function and Lymphocyte Proliferation in Spleen of Mice

作　　者：高青[1] 龙军[1] 简立信 简志伟 潘苏华[1] 

机构地区：[1]南京中医药大学,南京210009 [2]NULIFE国际(亚洲)有限公司,广东珠海519000

出　　处：《中国实验方剂学杂志》2013年第16期259-263,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏高校优势学科建设工程资助项目(ysxk-2010)

摘　　要：目的:研究复方蛹虫草颗粒对小鼠免疫功能的影响。方法:动物分正常组、盐酸左旋咪唑组、复方蛹虫草颗粒0.355,0.71,2.13,4.26,8.52,12.78 g.kg-1ig给药组,采用2,4-二硝基氟苯诱导的小鼠迟发型变态反应(DTH)以及碳粒廓清实验,研究复方蛹虫草颗粒对小鼠免疫功能的作用。采用四甲基氮唑盐(MTT)比色法研究复方蛹虫草颗粒含药血清对小鼠正常以及刀豆蛋白A(ConA)、脂多糖(LPS)诱导的脾淋巴细胞增殖的影响。结果:复方蛹虫草颗粒0.355,2.13 g.kg-1组耳肿胀程度显著高于对照组(P<0.01,P<0.05);复方蛹虫草颗粒0.355,0.71 g.kg-1组能提高小鼠碳吞噬指数(P<0.05);与空白血清组比较,复方蛹虫草颗粒含药血清显著提高正常小鼠脾淋巴细胞增殖(2.13 g.kg-1组,P<0.01,4.26,8.52,12.78 g.kg-1组,P<0.05);复方蛹虫草颗粒含药血清4.26 g.kg-1组能显著增加ConA刺激的脾淋巴细胞增殖(P<0.01);复方蛹虫草颗粒含药血清12.78 g.kg-1组能显著增加LPS刺激的脾淋巴细胞增殖(P<0.05)。结论:复方蛹虫草颗粒具有增强免疫功能的作用。Objective： To investigate the influence of compound cordyceps militaris granules on the immunity function of mice. Method： Mice were randomized into the normal group, levamisole hydrochloride group and compound cordyeeps militaris granules group with 0. 355, 0.71, 2. 13, 4.26, 8.52, 12.78g·kg^-1, and dinitrofluorobenzene induced delayed type hypersensitivity test （DTH） and carbon clearance test in vivo were employed to evaluate the immunologic function of mice. Compound cordyceps militaris granules containing serum on effects of lipopolysaccharide （LPS） and concanavalin A （ConA） induced lymphocyte proliferation was detected by MTT test. Result： The compound cordyceps militaris granules given by ig 0. 355 g·kg^-1 could significantly potentiate DTH in mice （P 〈0.01 ） , and 2. 13 g·kg^-1 of the granules was significantly enhanced the DTH in mice （P 〈0. 05）. Carbon clearance test showed that compound cordyceps militaris granules given by ig （0.71, 0. 355 g·kg^-1） could evidently improve the carbon clearance indexes （P 〈0.05）. Lymphocyte proliferation in 2. 13 g·kg^-1 compound cordyceps militaris containing serum group was significantly enhanced comparing with control group（P 〈 0.01 ）, and 4.26, 8.52, 12.78 g·kg^-1 compound cordyceps militaris containing serum group was significantly enhanced comparing with control group （P 〈 0. 05）. Compound cordyceps militaris containing serum 4.26 g·kg^-1 could enhance ConA-induced T cell proliferation （P 〈 0.01 ）. Compound cordyceps militaris contaming serum 12.78 g·kg^-1 could improve the proliferation of LPS-induced B lymphocyte obviously （P 〈 0.05）. Conclusion： Compound cordyceps militaris granules can enhance immune function obviously.

关 键 词：迟发型变态反应 碳廓清试验 淋巴细胞增殖 

分 类 号：R285.5[医药卫生—中药学]