枳实消痞丸诱导胃癌SGC-7901细胞凋亡的研究  

Studying of Apoptosis of SGC-7901 Cell Induced by Zhishi Xiaopi Wan

作　　者：吴耀松[1] 彭贵军[1] 

机构地区：[1]河南中医学院,郑州450046

出　　处：《中国实验方剂学杂志》2013年第16期278-281,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南中医学院苗圃工程项目

摘　　要：目的:从细胞增殖、凋亡和周期角度研究枳实消痞丸治疗胃癌的机制。方法:胃癌SGC-7901细胞阴性对照组加完全培养基,1×104细胞/孔接种于96孔板,将枳实消痞丸分为4,2,0.5,0.125,0.05 g.L-15个质量浓度加入,分别作用24,48,72 h,MTT法检测细胞增殖;1×105/孔接种于6孔板,加入2,0.5,0.125 g.L-13个质量浓度的药物,分别培养48,72 h,流式细胞仪检测细胞周期和细胞凋亡。结果:枳实消痞丸4,2,0.5,0.125,0.05 g.L-1质量浓度作用于胃癌SGC-7901细胞,抑制率依次降低;作用于24,48,72 h,抑制率渐增。枳实消痞丸增加了G0/G1期的细胞比率,促进细胞凋亡,高、中浓度强于低浓度。结论:枳实消痞丸抑制了细胞的增殖,具有时间和浓度依赖性,其机制可能与药物阻滞细胞于G0/G1期和诱导细胞凋亡有关。Objective： Study on the mechanism of Zhishi Xiaopi Wan treatment on Gastric cancer from cell proliferation, apoptosis and cycle. Method： Complete medium was mixed in negative control group; 1 × 10^4 cells/wells were seeded at 96 wells plate and divided into negative control, Zhishi Xiaopi Wan in 0.05, 0. 125, 0.5, 2, 4 g·L^-1 concentration groups; the cells were treated with drugs for 24, 48, 72 h, and cell proliferation was detected by MTT method; 1 × 10^5 cells/wells were seeded in 6 wells plate, and the drugs of low （0. 125 g·L^-1 ）, medium （0.5 g·L^-1 ）, .high （2 g·L^-1 ） concentration respectively wereput into wells for 48, 72 h, then the cell cycle and apoptosis of gastric cancer SGC-7901 cells were detected by flow cytometry （FCM）. Result： Gastric cancer SGC-7901 cells were treated by the 0. 125, 0.5, 2 g·L^-1 of Zhishi Xiaopi Wan, and its inhibition rate is in dose and time dependent. Zhishi Xiaopi Wan could increase SGC-7901 cells in G0-G1 phase percentage and promote apoptosis; The high, medium concentration was stronger than the lows. Conclusion： Zhishi Xiaopi Wan can inhibit the proliferation of SGC-7901 cell and there is in dose and time dependent, which mechanism may be related to drug block of cells to G0/G1 phase and induce cell apoptosis.

关 键 词：枳实消痞丸 SGC-7901细胞 细胞周期 凋亡 

分 类 号：R285.5[医药卫生—中药学]