关附壬素在Caco-2细胞上的摄取特性研究  

Uptake Determination of Guanfu Base I in Caco-2 Cells

作　　者：王瑞[1,2] 于彤[1] 褚延乐[1] 王琳茜[1] 李晓天[1] 

机构地区：[1]郑州大学药学院,郑州450001 [2]漯河市中心医院,河南漯河462000

出　　处：《中国实验方剂学杂志》2013年第16期282-285,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究关附壬素在Caco-2细胞上的摄取特性。方法:采用液相色谱质谱联用(LC-MS)测定法,COSMOSILC18柱(150 mm×2.0 mm,5μm),流动相:乙腈-水(含0.2%冰乙酸)30∶70,流速0.2 mL.min-1,电喷雾离子化(ES1)方式,采用选择性离子检测法,检测离子为正离子,关附壬素的选择性离子:[M+H],m/z 388。考察了温度、药物浓度、时间和pH对关附壬素在Caco-2细胞上摄取的影响。结果:关附壬素在0.2～50.0μmol.L-1内呈良好的线性关系(r=0.996 7),最低定量限为0.2μmol.L-1。关附壬素在Caco-2细胞上的摄取具有一定的浓度、时间和pH依赖性。结论:该方法灵敏、操作简单,选择性强,关附壬素在Caco-2细胞上摄取良好,其摄取表现为被动扩散。Objective： To study the uptake status of Guanfu Base I （GFI） in Caco-2 ceils. Method： To establish Liquid Chromotography with Mass Spectrometry （LC-MS） assay, A LC procedure was carried out using an C18 （ 150 mm × 2.0 mm, 5 μm） and an eluate of 0.2% acetic acid-acetonitrile （30：70） at the flow rate of O. 2 mL -min-1 The MS portion was set with ESI ionsource in the positive ion polarity mode and the selective ion monitoring （SIM）. A protonated molecules [ M ＋ H ] were detected at m/z 388.10 for GFI. The GFI concentrations in Caco-2 ceils at various time, concentration, temperature and pH values were measured. Result： The linearity of GFI concentrationcurve was found in the range of 0.2-50.0 μmol ·L^-1 （ r = 0. 996 7 ）. The minimal detection limit was 0.2 μmol ·L^-1 The GFI uptakes in Caco-2 cells obeyed a passive diffusion in a time, concentration and pH-dependent manner. Conclusion： This sensitive and specific LC-MS assay can be applied to determine GFI concentrations in Caco-2 cells.

关 键 词：关附壬素 LC—MS CACO-2细胞 摄取 

分 类 号：R285.5[医药卫生—中药学]