3种不同中医治法对大鼠肝脏癌细胞半乳糖凝集素表达的影响  

Effects of Three Different Chinese Medical Therapies on the Expression of Galectin in Rat Hepatoma Cells

作　　者：胡卫[1] 方肇勤[2] 梁超[2] 管冬元[2] 吴中华[2] 

机构地区：[1]三峡大学医学院,湖北省宜昌市大学路8号443002 [2]上海中医药大学基础医学院

出　　处：《中医杂志》2013年第16期1401-1403,1425,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30672574;30973703)

摘　　要：目的观察3种不同中医治法对大鼠肝癌半乳糖凝集素(GAL-3)表达的调控差异以及GAL-3在人肝癌细胞增殖中的作用,以探讨肝癌治疗的新靶点。方法将72只雄性Wistar大鼠随机分为正常组、模型组、喃氟啶组(喃氟啶8.33mg/100g)、清热解毒组(清热解毒方1.5g/100g)、活血化瘀组(活血化瘀方1.27g/100g)、健脾理气组(健脾理气方1.75g/100g),每组12只,除正常组外,其余各组大鼠采用二乙基亚硝胺(DEN)诱发大鼠肝癌,各组均给药6周,检测各组大鼠肝癌组织(正常组取肝组织)GAL-3的表达。根据人GAL-3基因编码区设计2个siRNA靶点,并构建重组质粒转染SMMC-7721人肝癌细胞株,分为空白组、阴性组、GAL-3RNAi组,实时定量PCR检测各组GAL-3mRNA的表达,MTT法检测各组转染后24h、72h、144h细胞增殖。结果模型组GAL-3基因表达显著增加,健脾理气组能下调其表达;转染72h后GAL-3RNAi组GAL-3mRNA表达量明显降低;转染144h后,GAL-3RNAi组肝癌细胞的生长增殖得到了明显的抑制(P<0.01)。结论 GAL-3基因在肝癌组织中高表达,健脾理气方能显著下调该基因表达。Objective To observe the effect of different Chinese medical therapies on the expression and proliferation of Galectin-3（GAL-3） in human hepatoma cells.Methods Seventy-two male Wistar rats were randomized into the normal group,model group,Tegafur group（8.33 mg/100 g Tegafur）,Heat-Clearing Toxin-Resolving Formula group（1.5 g/100 g HCTRF）,Blood-Quickening Stasis-Transforming Formula group（1.27 g/100 g BQSTF） and Spleen-Fortifying Qi-Regulating Formula group（1.75 g/100 g SFQRF）,with 12 in each.Diethylnitrosamine（DEN） was used to induce the hepatic carcinoma rat models in each group except the normal group.The rats in the administration groups were gavaged 12 times daily for six weeks.The expression of GAL-3 in the rat hepatocellular carcinoma（HCC） tissues（liver tissues in the normal group） was detected.Two siRNA targets were designed according to human GAL-3 gene coding region and the recombinant plasmid transfection human hepatoma cell line SMMC-7721 was constructed.The gene expression was detected by real-time quantitative polymerase chain reaction（PCR） and the cell proliferation was detected by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assay 24 h,72 h,and 144 h after transfection.Results The expression of GAL-3 gene in the model group was significantly increased.SFQRF could down-regulate the expression of GAL-3 gene,the expression of GAL-3 mRNA was significantly decreased 72 h after transfection and the proliferation of hepatoma cells was significantly inhibited 144 h after transfection（P〈0.01）.Conclusion The GAL-3 gene is highly expressed in HCC tissues and SFQRF can significantly down-regulate its expression.

关 键 词：GAL-3基因 肝癌 健脾理气方 清热解毒方 活血化瘀方 细胞增殖 

分 类 号：R273[医药卫生—中西医结合]