1株番鸭源新型鸭呼肠孤病毒(QY株)生物学鉴定  

Biological identification of a new-type duck reovirus(QY strains)isolated from Muscovy duck

作　　者：袁远华[1,2] 王俊峰 吴志新 黄兴国[2] 贺东生[1] 黄淑坚[2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]佛山科学技术学院生命科学学院,广东佛山528231 [3]韶关市动物卫生监督所,广东韶关512026 [4]广东佛山三水区乐平镇动物防疫检疫站,广东佛山528137

出　　处：《中国兽医学报》2013年第8期1174-1178,共5页Chinese Journal of Veterinary Science

基　　金：广东省科技厅农业攻关资助项目(2012A020100001)

摘　　要：从广东清远地区临床病变为发病鸭软脚,肝、脾肿大,有点/块状出血、坏死,其他各脏器不同程度出血的病死雏番鸭肝、脾组织中分离到1株病毒。通过形态观察、核酸图谱鉴定、RT-PCR扩增、基因序列分析、动物回归试验结果表明,该病毒为一株不同于以往番鸭呼肠孤病毒的新型鸭呼肠孤病毒,并命名为DRV-QY株。A virus was isolated from the liver and spleen of the infected cheeper Muscovy ducks in Guangdong Province. The sick ducks showed weakness in legs and pathological findings included hemorrhage necrosis in the spleen and liver. The virus isolate designated was demonstrated differ-ent from those previously documented in pathogenecity in ducks,virus morphology under electron microscopy,SDS-PAGE analysis of the viral genome and genetic analysis by RT-PCR. Thus, the virus isolate designated was a new-type duck reovirus,and was named DRV-QY strains.

关 键 词：番鸭 鸭呼肠孤病毒 生物学鉴定 

分 类 号：S852.65[农业科学—基础兽医学]