Ⅰ群禽腺病毒血清10型的致病性  

Pathogenicity of fowl adenovirusesⅠserotypeⅩ

作　　者：国纪垒[1] 刁有祥[1] 程彦丽[1] 宋晓娜[1] 张坤[1] 肖坡[1] 杨建朋[1] 孙晓艳[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医学报》2013年第8期1179-1183,共5页Chinese Journal of Veterinary Science

基　　金：现代农业产业技术体系专项资金资助项目(CARS-43-34)

摘　　要：将Ⅰ群禽腺病毒血清10型山东分离株(SDXT株)通过皮下注射接种途径人工感染1日龄SPF雏鸡,观察试验鸡的发病情况及临床症状;于感染后不同时间随机剖杀试验组和对照组鸡只,采集心脏、肝脏、肺脏和肠道等组织固定、切片、H.E.染色,观察各器官病理组织学变化,并进行血常规和血液生化指标检测。同时,应用IFA对SDXT株人工感染雏鸡的各组织器官进行检测。结果表明,SDXT株接种后,引起雏鸡精神沉郁、食欲不振、嗜睡等症状。病理组织学变化以肝脏脂肪变性、肝脏结构紊乱、肝窦间出血和炎性细胞浸润,在肝细胞核内可见嗜碱性包涵体为主;肠道内肠绒毛脱落;肺脏广泛性出血和炎性细胞浸润为特征。IFA检测结果显示,肝脏、脾脏、肠道、肺脏均可检测到SDXT株,其中肝脏和肠道检出率最高,表明这2个器官是其主要靶器官。HGB、WBC、RBC、ALT和ALP发生显著变化。结果表明,Ⅰ群禽腺病毒血清10型对雏鸡有较强的致病性。SPF chicks were taneous injection route to experimentally inoculated with one FAV-Ⅰ , serotype Ⅹ through subcu-observe incidence and clinical symptoms. The chicks of experimental group and the negative control group were sacrificed randomly after infection at presetted times. The heart,liver, lungs and intestine were collected,fixed and stained by conventional H. E. stai-ning. Then the pathological changes of these organs were observed, and hematological and bio-chemical parameters were evaluated. The chicks showed depression, anorexia, drowsiness and other symptoms after inoculation. Histopathological changes were characterized by fatty degeneration, extensive hemorrhage and inflammatory cells infiltration in the liver, basophilia inclusion body in the liver cells, shedding of intestinal villus in the duodenum, catholicity hemorrhage in the lungs. IFA showed that the stronger positive signals could be detected in intestine and liver which was considered to be target organs of FAV-Ⅰ. The level of HGB,WBC,RBC,BUN,and AST changed significantly. The results showed that the FAV-Ⅰ caused high pathogenicity to SPF chickens.

关 键 词：Ⅰ群禽腺病毒 SPF雏鸡 致病性 SPF雏鸡 

分 类 号：S852.65[农业科学—基础兽医学]