髓细胞瘤型J亚群禽白血病病毒四川株SCGS-1的分离鉴定及前病毒cDNA重组质粒的构建  

Isolation and identification of SCGS-1strain of subgroup J avian myeloid leukosis virus from Sichuan area and construction of full-length recombinat plasmid of pro viral cDNA

作　　者：林艳[1] 赵扬[1] 夏静[1] 邹年丽[1] 文心田[1,2] 曹三杰[1,2] 黄勇[1,2] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]四川农业大学动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国兽医学报》2013年第8期1190-1195,共6页Chinese Journal of Veterinary Science

基　　金：教育部<长江学者和创新团队发展计划>创新团队资助项目(IRTO848)

摘　　要：为分析髓细胞瘤型J亚群禽白血病病毒（ALV-J）地方分离毒株的分子特征及开展该病毒的反向遗传操作研究，本试验从四川某肉种鸡场髓细胞瘤病变病鸡的组织中分离到1株髓细胞瘤型ALV-J，命名为SCGS-1。序列测定表明其前病毒cDNA全序列长7499bp，与其他ALV—J代表毒株序列相似性均为95％～99％。在所有基因中，其env基因和LTR序列与其他毒株相应序列同源性相对较低，env基因同源性为93．0％～99．5％，LTR基因同源性为92％～95％，p01基因同源性为97％～990．4，gag基因同源性为94％～97％。根据该毒株序列和质粒pUC19的序列，将SCGS-1株前病毒eDNA全序列分3段克隆入pUC19中，构建含SCGS-1前病毒cDNA的重组载体并命名为pUC-SCGS。试验结果为研究ALV—J的进化规律和开展反向遗传操作打下基础。In order to investigate the molecular characteristice and to carry out the reverse genetics of avian myelocytomatosis of local strains. A isolate of subgroup J avian leukosis virus （ALV-J） with myelocytomatosis, designed SCGS-I,was isolated from grandparent meat chickens in Sichuan province. The viral genome of this isolate was sequenced as 7 499 bp and compared with reference ALV-J strains in GenBank. The results showed that the sequence identity was ranged from 95 % to 99%. Env gene and LTR were variated greater than pol and gag gene. The sequence identity was ranged from 93.0% to 99.5% for env gene and 92% to 95% for LTR,lower than 97% to 99% for pol gene and 94% to 97% for gag gene. A full-length recombinat plasmid （pUC-SCGS） was con-structed by cloning and combining of three fragments using PCR method depended on the sequence of SCGS-1 and pUC19. The results may contribute to study the evolution and reverse genetics of ALV-J.

关 键 词：髓细胞瘤 ALV—J 肉鸡 

分 类 号：S852.65[农业科学—基础兽医学]