玉米赤霉烯酮中毒大鼠卵巢组织p53和NF-κB的表达  

Expression of p53and NF-κB of ovaries in SD rats with zearalenone mycotoxicosis

作　　者：周宏超[1] 郭良[1] 史嘉瑜[1] 赵煜[1] 刘晓强[1] 杨鸣琦[1] 李引乾[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《中国兽医学报》2013年第8期1278-1281,共4页Chinese Journal of Veterinary Science

基　　金：陕西省科技攻关资助项目(2009K02-01)

摘　　要：10周龄SD大鼠单剂量腹腔注射5mg/kg玉米赤霉烯酮(ZEA)玉米油溶液,分别于攻毒后3,6,12,24,48,72,96h剖杀取卵巢组织,检测不同时间卵巢组织中p53和NF-κB的表达。病理组织学观察发现卵巢组织出现不同程度损伤,卵泡颗粒细胞凋亡。免疫组化结果表明试验组与对照组卵巢组织中均有p53和NF-κB的表达,并且随着时间的推进呈现动态变化。试验组p53在3,6,12h时表达量上调,12h后表达量逐渐下降,各试验组与对照组相比较均差异显著(P<0.05)。NF-κB在3h表达量最高,而后表达量开始降低,各试验组与对照组相比较差异均显著(P<0.05)。大鼠玉米赤霉烯酮中毒可引起卵巢组织的病变及颗粒细胞凋亡,且p53和NF-κB在玉米赤霉烯酮中毒大鼠卵巢颗粒细胞凋亡发生过程中起着一定作用。The objective of this study was to determine the expression of p53 and NF-κB in the ova- ries of SD rats exposed to zearalenone（ZEA）. Ten-week-old SD rats which were used to determine the expression of p53 and NF-κB were treated with 5 mg/kg i. p. of ZEA and euthanized at 3,6,12, 24,48,72 and 96 h after treatment. Histopathologically, ovarian follicles were affected selectively and apotosis cell were found in granular layer in ovarian follicle. Immunohistochemic analysis re-vealed the expression of p53 and NF-κB in experiment groups and control groups,with dynamic changes over time. p53 expression increased at 3,6,12 h and reduced after 12 h of treatment com-pared to control groups（P〈0.05）. In all test group,NF-κB increased at 3 h and reduced after 3 h, compared to control groups（P〈0.05）. Zearalenone mycotoxicosis induced ovarian follicles damage and granulosa cell apotosis in rats. p53 and NF-κB lay an important role in apoptosis of granulosa cells in the rats with zearalenone mycotoxicosis.

关 键 词：玉米赤霉烯酮 卵巢颗粒细胞 P53 NF-ΚB 大鼠 

分 类 号：S856.9[农业科学—临床兽医学] S859.87[农业科学—兽医学]