不同能量、蛋白水平日粮对育雏期塞北乌骨鸡生长发育规律、生化指标和肠道消化酶的影响  

Effect of different energy and protein levels in diet on the growth performance,bi ochemistry index and intestianal digestive enzyme of Saibei silkies in starting period

作　　者：官丽辉[1] 刘海斌[1] 张立永[1] 刘立文[1] 

机构地区：[1]河北北方学院动物科技学院,河北张家口075131

出　　处：《中国兽医学报》2013年第8期1292-1300,共9页Chinese Journal of Veterinary Science

基　　金：河北省科学技术厅科技攻关资助项目(02220402D);河北省教育厅支撑计划资助项目(Z2007427);河北省高校百名优秀创新人才支持计划资助项目(BR2-106);河北北方学院课题资助项目(2007013)

摘　　要：900只1日龄塞北乌骨鸡采用5（能量）×2（蛋白）两因素配方设计，试验期6周。结果：6周龄母鸡日粮代谢能（ME）为12．65MJ／kg，体增重最高；公鸡日粮ME为12．00MJ／kg，体增重最高。不同能量、蛋白水平日粮对21和42E1龄塞北乌骨鸡血液生化指标存在不同程度的影响，高能量日粮可显著提高血清中血糖、总胆固醇和甘油三酯的含量（P〈0．05）。在ME为12．65MJ／kg、蛋白为20％的日粮，3周龄母鸡的净体质量和肝质量极显著大于公鸡（P〈0．01），消化道质量公鸡极显著大于母鸡（P〈0．01）。在ME为12．65MJ／kg、蛋白为18％的日粮母鸡的净体质量极显著小于公鸡（P〈0．01）；心质量显著小于公鸡（P〈0．05）；消化道质量公鸡极显著大于母鸡（P〈0．01）。0～6周龄消化道发育公鸡显著快于母鸡，但消化道总长变化不明显（P〉0．05）。消化道总长与消化道各段长度的回归方程的决定系数（R^2）均超过了0．807，总体效果公鸡略优于母鸡。21日龄时随着日粮能量水平的提高肠道胰蛋白酶活性除ME为10．85MJ／kg与12．00MJ／kg组差异显著外（P〈0．05），公鸡其他各组间差异均不显著；母鸡在ME为12．65MJ／kg时最高。淀粉酶和胰脂肪酶不同能量间差异均不显著（P〉0．05）。42日龄公鸡脂肪酶活性和胰蛋白酶活性都不显著（P〉0．05）；母鸡从总体来看，肠道消化酶活性随着日粮能量水平的提高而增加，日粮ME为12．65MJ／kg达到最高。A 5 × 2 factorial experiment containing five ME levels （10. 60, 10. 85,11. 51,12. 00, 12.65 MJ/kg） and two CP levels （18%,20%） was conducted to investigate effects of energy and protein levels on growth performance, biochemistry index and intestinal digestive enzyme. The re-sults showed that：when ME was 12.65 MJ/kg and 12.00 MJ/kg,body gain of female and male was biggest;effects of different ME and CP on Saibei silkies biochemistry index at 21 days and 42 days were different,high enery level in diet significantly improved glucose,cholesterol and triglyc-eride（P〈0.05） ;body weight and liver weight of female at 3 weeks were significantly bigger than male（P〈0.01） ,but enteron weight of male were bigger than female when ME was 12.65 MJ/kg and CP was 20% （P〈0.01）. Body weight of female were bigger than male（P〈0.01）, heart weight was bigger than male when ME was 12.65 MJ/kg and CP was 18% （P〈0.05）. The R2of total en-teron length and every part length was bigger than 0. 807. There was no significantly effects on male protease at 21 days（P〈0.05） except ME was 10.85 MJ/kg and 12.00 MJ/kg,protease of fe-male at 42 days were biggest when ME was 12.65 MJ/kg（P〉0.05）. Protease and lipase of male were not significant; intestianal digestive enzyme of femal were improved by ME levele, it was biggest when ME was 12.65 MJ/kg.

关 键 词：塞北乌骨鸡 能量与蛋白水平 生长发育规律 生化指标 肠道消化酶 

分 类 号：S816[农业科学—饲料科学]