神农架川金丝猴粪便和尿液中类固醇激素的检测  

Measurement of urinary and fecal steroid metabolites in a provisioned group of golden snub-nosed monkeys(Rhinopithecus roxellana)at Shennongjia Reserve,Hubei,China

作　　者：范鹏来[1] 陈浩春[1] 姚辉 王振龙[3] 杨敬元 向左甫[1] 

机构地区：[1]中南林业科技大学生命科学与技术学院,长沙410004 [2]湖北神农架金丝猴保育生物学湖北省重点实验室,神农架442411 [3]郑州大学生物多样性与生态学研究所,郑州450001

出　　处：《兽类学报》2013年第3期286-292,共7页Acta Theriologica Sinica

基　　金：国家科技支撑计划(2013BAD03-02);国家林业局野生动物保护与繁育项目;湖南省教育厅创新平台开放项目(11K078);湖北省重点实验室开放基金支持项目(2011SNJSYS002)

摘　　要：灵长类动物类固醇激素的研究已经在很多物种间展开（Cariso et al.,1999;Lutz et al.,2000;He et al.,2001;Yan and Jiang,2006;Brandon et al.,2008;Lu et al.,2010;Kim et al.,2012）。特别是对于没有明显发情特征的灵长类动物,类固醇激素的变化可以为它们的繁殖状态提供更多可靠的评估（Fujita et al.,2001）。Steroid hormone metabolites in the feces and urine（SHMFU）have been used to monitor the physiological status,i.e.,reproductive,health or social status,in many primate species.Owing to the different metabolic path in the body,SHMFU may have different concentration peak.The refore,it is necessary to analyze the correlation or consistency of SHMFU when apply them to identify the physiological status of the animal.In this study,we clarify the consistency of SHMFU of a semi-free ranging group in golden snub-nosed monkeys（Rhinopithecus roxellana）at Shennongjia,central China,with total 84 samples,including two adult males（24 samples）and ten adult females（60 samples）,from November,2011 to June,2012.Enzyme-linked immunoassays（EIA）were applied to detect the testosterone of the male,testosterone estradiol and progesterone of the female.Significant correlation of testosterone,testosterone estradiol and progesterone in the feces and urine were found both the males and females.The result indicated that it was feasible to judge the physiological status of R.roxellana through SHMFU.However,this study couldn＇t provide the lag time of SHMFU because the number of samples is not enough.

关 键 词：川金丝猴 类固醇激素 一致性 酶联免疫吸附测定法 

分 类 号：Q958[生物学—动物学]